Team:Hong Kong HKUST/pneumosensor/parts

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<a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor">Pneumosensor</a>
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<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/module_one">Sensing</a></li>
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<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/characterization">Characterization</a></li>
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<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/parts">Parts</a></li>
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<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/data">Data</a></li>
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<li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/start-up_kit">Start-up Kit</a></li>
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<li class= "indent_list"><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/start-up_kit/handbook" >--Handbook</a></li>
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<li class= "indent_list"><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/start-up_kit/report" >--Report</a></li>
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<h2>Pneumosensor Parts</h2>
<h2>Pneumosensor Parts</h2>
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<p>This page shows all the parts we have made or used in our whole project. Click on the links below to see our constructs on the Registry of Standard Biological Parts in detail. Click <a href= “https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/results”> here </a>to access our result page for an overview of our achievements with those parts.</p>
 
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<br>
 
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How our system works:
 
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[Diagram with Biobrick Code]
 
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<br>
 
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<b>Data For Our Favorite New Parts:</b>
 
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<ul style="font-size: 20px">
 
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<li> <b> Group 1/2???</b>
 
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<li> <b>&sigma;<sup>x</sup>, <a href= “http://parts.igem.org/Part:BBa_K1379004”> BBa_K1379004 </a> </b>: gene sequence of competence protein comX which produces &sigma;<sup>x</sup> factor. &sigma;<sup>x</sup> factor induces promoter containing combox sequence (P<sub>celA</sub> and P<sub>helicase</sub>).
 
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<li> <b>P<sub>celA</sub> promoter, <a href= “http://parts.igem.org/Part:BBa_K1379000”> BBa_K1379000 </a></b>: a &sigma;<sup>x</sup>-inducible promoter (containing combox sequence) which initiates the transcription of competence CelA protein.
 
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<li> <b>P<sub>helicase</sub> promoter, <a href= “http://parts.igem.org/Part:BBa_K1379001”> BBa_K1379001 </a> </b>: a &sigma;<sup>x</sup>-inducible promoter (containing combox sequence) which initiates the transcription of helicase protein.
 
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<b>Data for Pre-existing Parts:</b>
 
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<li> <b> Group 1/2???</b>
 
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<li><b>Strong promoter, strong RBS combination, <a href= “http://parts.igem.org/Part:BBa_K880005”> BBa_K880005 </a></b>(Team Michigan iGEM, 2012) : combination of strong constitutive promoter (<a href= “http://parts.igem.org/wiki/index.php?title=Part:BBa_J23100”> BBa_J23100</a>) and strong RBS (<a href= “http://parts.igem.org/wiki/index.php?title=Part:BBa_B0034”> BBa_B0034 </a>) was used as a backbone for a very high level of expression of ComX protein (&sigma;<sup>x</sup> factor).
 
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<li><b>GFP generator</b>, <a href= “http://parts.igem.org/Part:BBa_E0240”> BBa_E0240 </a> </b>(Antiquity Boston University iGEM, 2004) : green fluorescent protein reporter (<a href= “http://parts.igem.org/Part:BBa_E0240”> BBa_E0040 </a>) with RBS (<a href= “http://parts.igem.org/Part:BBa_B0032”> BBa_B0032</a>) and double terminator (<a href= “http://parts.igem.org/Part:BBa_B0015”>  BBa_B0015</a>) was used as a reporter for characterization of P<sub>celA</sub> and P<sub>helicase</sub> promoters.
 
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<b>We Also Built and Characterized the Following Parts</b>
 
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<li><b>Group1/2???</b>
 
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<li> <b>&sigma;<sup>x</sup> Generator, <a href= “http://parts.igem.org/Part:BBa_K1379006”> BBa_K1379006 </a> </b> : <i>comX</i> gene sequence was assembled with constitutive promoter, RBS and double terminator so ComX protein is produced constitutively.
 
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<li> <b> P<sub>celA</sub>-GFP Generator, <a href= “http://parts.igem.org/Part:BBa_K1379002”> BBa_K1379002 </a></b>: &sigma;<sup>x</sup>-inducible P<sub>celA</sub> promoter was assembled with GFP generator.
 
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<li> <b>P<sub>helicase</sub>-GFP Generator, <a href= “http://parts.igem.org/Part:BBa_K1379003”> BBa_K1379003</a> </b> : &sigma;<sup>x</sup>-inducible P<sub>helicase</sub> promoter was assembled with GFP generator.
 
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<li> <b> &sigma;<sup>x</sup> Generator, P<sub>celA</sub>-GFP Generator, <a href= “http://parts.igem.org/Part:BBa_K1379005”> BBa_K1379005 </a></b>: &sigma;<sup>x</sup>-generator was assembled with P<sub>celA</sub>-GFP generator. Following the assemblage of both constructs, GFP was successfully expressed.
 
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<li> <b>&sigma;<sup>x</sup> Generator, P<sub>helicase</suB>-GFP Generator, <a href= “http://parts.igem.org/Part:BBa_K1379007”> BBa_K1379007 </a> </b>: &sigma;<sup>x</sup>-generator was assembled with P<sub>helicase</sub>-GFP generator. Following the assemblage of both constructs, GFP was successfully expressed.
 
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<td class= 'site_map_column'><h4><a href="https://2014.igem.org/Team:Hong_Kong_HKUST">Home</a></h4>
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<li class='site_link'><a href="">Pneumosensor</a></li>
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<li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice">Human Practice</a></li>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379004">BBa_K1379004</a></td>  
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  <td>Coding</td>    
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  <td>σ<sup>x</sup></td>  
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  <td>513</td>
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<td class= 'site_map_column'><h4><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor">Pneumosensor</a></h4>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379006">BBa_K1379006</a></td>  
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  <td>Generator</td>    
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<li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/module_one">Sensing</a></li>
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  <td>σ<sup>x</sup> Generator</td>  
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<li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/module_two">Expressing</a></li>
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  <td>711</td>
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<li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/parts">Parts</a></li>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379002">BBa_K1379002</a></td>  
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  <td>Measurement</td>    
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  <td>P<sub>celA</sub>-E0240 (Measurement Kit for P<sub>celA</sub>)</td>  
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  <td>984</td>
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<li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/parts">Parts</a></li>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379003">BBa_K1379003</a></td>  
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  <td>Measurement</td>    
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<li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/characterization">Characterization</a></li>
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  <td>P<sub>comFA</sub>-E0240 (Measurement Kit for P<sub>comFA</sub>)</td>  
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<li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/results">Results</a></li>
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  <td>1044</td>
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<li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/wetlab/protocols">Protocols</a></li>
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<li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/wetlab/safety">Safety</a></li>
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</ul>
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</td>
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<td class= 'site_map_column'><h4><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/Achievements">Achievement</a></h4>
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<ul class= 'site_list'>
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<li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/acheivement/medal_requirement">Medal Requirements</a></li>
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<li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/acheivement/deliverable">Deliverable</a></li>
+
-
</ul>
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-
</td>
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-
</tr>
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<tr>
-
+
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379045">BBa_K1379045</a></td>
-
</table>
+
  <td>Composite</td>     
 +
  <td>σ<sup>x</sup> + B0015</td>  
 +
  <td>650</td>
 +
</tr>  
 +
 +
<tr>
 +
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379000">BBa_K1379000</a></td>
 +
  <td>Regulatory</td>     
 +
  <td>P<sub>celA</sub> 100 basepairs combox promoter</td>  
 +
  <td>100</td>
 +
</tr>
 +
 +
<tr>
 +
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379001">BBa_K1379001</a></td>
 +
  <td>Regulatory</sub></td>     
 +
  <td>P<sub>comFA</sub> 160 base pairs combox promoter</td>  
 +
  <td>160</td>
 +
</tr>
 +
 +
                                <tr>
 +
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379005">BBa_K1379005</a></td>
 +
  <td>Measurement</td>     
 +
  <td>σ<sup>x</sup> + P<sub>celA</sub>-E0240</td>  
 +
  <td>1703</td>
 +
</tr>
 +
 
 +
<tr>
 +
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379007">BBa_K1379007</a></td>
 +
  <td>Measurement</td>     
 +
  <td>σ<sup>x</sup> + P<sub>comFA</sub>-E0240</td>  
 +
  <td>1763</td>
 +
</tr>
 +
 
 +
<tr>
 +
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379051">BBa_K1379051</a></td>
 +
  <td>Coding</td>     
 +
  <td><i>comE</i></td>  
 +
  <td>753</td>
 +
</tr>
 +
 
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</tbody>
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</table>
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Latest revision as of 18:19, 17 October 2014



Pneumosensor Parts

Name Type Description Length
BBa_K1379004 Coding σx 513
BBa_K1379006 Generator σx Generator 711
BBa_K1379002 Measurement PcelA-E0240 (Measurement Kit for PcelA) 984
BBa_K1379003 Measurement PcomFA-E0240 (Measurement Kit for PcomFA) 1044
BBa_K1379045 Composite σx + B0015 650
BBa_K1379000 Regulatory PcelA 100 basepairs combox promoter 100
BBa_K1379001 Regulatory PcomFA 160 base pairs combox promoter 160
BBa_K1379005 Measurement σx + PcelA-E0240 1703
BBa_K1379007 Measurement σx + PcomFA-E0240 1763
BBa_K1379051 Coding comE 753

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