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- | <nav role="navigation">
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- | <ul class="access-menu">
| + | <div id="content_container"> |
- | <li><a href="#">Home</a></li>
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- | <li>
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- | <a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor">Pneumosensor</a>
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- | <ul class="access-submenu">
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- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/module_one">Sensing</a></li>
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- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/module_two">Expressing</a></li>
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- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/parts">Parts</a></li>
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- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/data">Data</a></li>
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- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/characterization">Characterization</a></li>
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- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/results">Results</a></li>
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- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/future_work">Future Work</a></li>
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- | </ul>
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- | </li>
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- | <li>
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- | <a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator">Riboregulator</a>
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- | <ul class="access-submenu">
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- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/CR_TA_Feature_Page">Feature Page</a></li>
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- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/RNA_devices_catalog">Catalog Page</a></li>
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- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/parts">Parts</a></li>
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- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/data">Data</a></li>
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- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/characterization">Characterization</a></li>
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- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/results">Results</a></li>
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- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/future_work">Future Work</a></li>
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- | </ul>
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- | </li>
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- | <li>
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- | <a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice">Human Practice</a>
| + | |
- | <ul class="access-submenu">
| + | |
- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/start-up_kit">Start-up Kit</a></li>
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- | <li class= "indent_list"><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/start-up_kit/handbook" >--Handbook</a></li>
| + | |
- | <li class= "indent_list"><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/start-up_kit/report" >--Report</a></li>
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- | <li class= "indent_list"><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/start-up_kit/database" >--Database</a></li>
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- | <li class= "indent_list"><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/start-up_kit/interview" >--Interview</a></li>
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- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/outreach">Outreach</a></li>
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- | <li class= "indent_list"><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/outreach/Workshop" >--Workshop</a></li>
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- | <li class= "indent_list"><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/outreach/talks" >--Talk</a></li>
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- | <li class= "indent_list"><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/outreach/isf_academy" >--ISF Academy</a></li>
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- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/safety_and_ethics">Safety and Ethics</a></li>
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- | </ul>
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- | </li>
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- | <li>
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- | <a href="https://2014.igem.org/Team:Hong_Kong_HKUST/team">Team</a>
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- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/team/members">Members</a></li>
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- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/team/advisers">Advisers</a></li>
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- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/team/instructors">Instructors</a></li>
| + | |
- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/team/attribution">Attributions</a></li>
| + | |
- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/team/acknowledgement">Acknowledgement</a></li>
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- | <li><a href="https://igem.org/Team.cgi?year=2014">Official Team Profile</a></li>
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- | </ul>
| + | |
- | </li>
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- | <li>
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- | <a href="https://2014.igem.org/Team:Hong_Kong_HKUST/wetlab">Wetlab</a>
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- | <ul class="access-submenu">
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- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/wetlab/notebook">Notebook</a></li>
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- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/wetlab/protocols">Protocols</a></li>
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- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/wetlab/safety">Safety</a></li>
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- | </ul>
| + | |
- | </li>
| + | |
- | <li>
| + | |
- | <a href="https://2014.igem.org/Team:Hong_Kong_HKUST/Achievements">Achievements</a>
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- | <ul class="access-submenu">
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- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/acheivement/medal_requirement">Medal Requirements</a></li>
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- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/acheivement/deliverable">Deliverable</a></li>
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- | </ul>
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- | </li>
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- | <li class="access_logo">
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- | <a href="https://2014.igem.org/Main_Page"><img src= "https://static.igem.org/mediawiki/2014/5/55/Hkust_logo.gif"></a>
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- | </li>
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- | <a href="http://www.ust.hk/"><img src= "https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"></a>
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- | <div class= "banner_area">
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- | <img src= 'https://static.igem.org/mediawiki/2014/archive/3/3c/20140930022303!HKUST_2014_pneumosensor_banner.jpg' />
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| <div id="description_area"> | | <div id="description_area"> |
| <h2>Pneumosensor Parts</h2> | | <h2>Pneumosensor Parts</h2> |
- | <p>This page shows all the parts we have made or used in our whole project. Click on the links below to see our constructs on the Registry of Standard Biological Parts in detail. Click <a href= “https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/results”> here </a>to access our result page for an overview of our achievements with those parts.</p>
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- | </div>
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- | <hr>
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- | <div class='content_1'>
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- | <table class="content_table" align= "center" >
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- | <tr class= "content_row" >
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- | <td class= "content_cell" colspan= "2">
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- | <div class= "content_area_two_row">
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- | <p>
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- | <br>
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- | How our system works:
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- | [Diagram with Biobrick Code]
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| | | |
- | <p>
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- | <br>
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- | <b>Data For Our Favorite New Parts:</b>
| |
- | <ul>
| |
- | <li> <b> Group 1/2???</b>
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- | <li> <b>σ<sup>x</sup>, <a href= “http://parts.igem.org/Part:BBa_K1379004”> BBa_K1379004 </a> </b>: gene sequence of competence protein comX which produces σ<sup>x</sup> factor. σ<sup>x</sup> factor induces promoter containing combox sequence (P<sub>celA</sub> and P<sub>helicase</sub>).
| |
- | <li> <b>P<sub>celA</sub> promoter, <a href= “http://parts.igem.org/Part:BBa_K1379000”> BBa_K1379000 </a></b>: a σ<sup>x</sup>-inducible promoter (containing combox sequence) which initiates the transcription of competence CelA protein.
| |
- | <li> <b>P<sub>helicase</sub> promoter, <a href= “http://parts.igem.org/Part:BBa_K1379001”> BBa_K1379001 </a> </b>: a σ<sup>x</sup>-inducible promoter (containing combox sequence) which initiates the transcription of helicase protein.
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- | </ul>
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- | </p>
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| | | |
- | <br>
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- | <br>
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- | <p>
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- | <br>
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- | <b>Data for Pre-existing Parts:</b>
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- | <ul>
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- | <li> <b> Group 1/2???</b>
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- | <li><b>Strong promoter, strong RBS combination</b>, <a href= “http://parts.igem.org/Part:BBa_K880005”> BBa_K880005 </a></b>(Team Michigan iGEM, 2012) : combination of strong constitutive promoter (<a href= “http://parts.igem.org/wiki/index.php?title=Part:BBa_J23100”> BBa_J23100</a>) and strong RBS (<a href= “http://parts.igem.org/wiki/index.php?title=Part:BBa_B0034”> BBa_B0034 </a>) was used as a backbone for a very high level of expression of ComX protein (σ<sup>x</sup>factor).
| |
- | <li><b>GFP generator</b>, <a href= “http://parts.igem.org/Part:BBa_E0240”> BBa_E0240 </a> </b>(Antiquity Boston University iGEM, 2004) : green fluorescent protein reporter (<a href= “http://parts.igem.org/Part:BBa_E0240”> BBa_E0040 </a>) with RBS (<a href= “http://parts.igem.org/Part:BBa_B0032”> BBa_B0032</a>) and double terminator (<a href= “http://parts.igem.org/Part:BBa_B0015”> BBa_B0015</a>) was used as a reporter for characterization of P<sub>celA</sub> and P<sub>helicase</sub> promoters.
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- | </ul>
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- | <p>
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- | <br>
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- | <br>
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- | <p>
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- | <br>
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- | <b>We Also Built and Characterized the Following Parts</b>
| |
- | <ul>
| |
- | <li><b>Group1/2???</b>
| |
- | <li> <b>σ<sup>x</sup> Generator, <a href= “http://parts.igem.org/Part:BBa_K1379006”> BBa_K1379006 </a> </b> : <i>comX</i> gene sequence was assembled with constitutive promoter, RBS and double terminator so ComX protein is produced constitutively.
| |
- | <li> <b> P<sub>celA</sub>-GFP Generator, <a href= “http://parts.igem.org/Part:BBa_K1379002”> BBa_K1379002 </a>: σ<sup>x</sup>-inducible P<sub>celA</sub>promoter was assembled with GFP generator.
| |
- | <li> <b>P<sub>helicase</sub>-GFP Generator, <a href= “http://parts.igem.org/Part:BBa_K1379003”> BBa_K1379003</a></b> : σ<sup>x</sup>-inducible P<sub>helicase</sub> promoter was assembled with GFP generator.
| |
- | <li> <b> σ<sup>x</sup> Generator, P<sub>celA</sub>-GFP Generator, <a href= “http://parts.igem.org/Part:BBa_K1379005”> BBa_K1379005 </a></b>: σ<sup>x</sup>-generator was assembled with P<sub>celA</sub>-GFP generator. Following the assemblage of both constructs, GFP was successfully expressed.
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- | <li> <b>σ<sup>x</sup> Generator, P<sub>helicase</suB>-GFP Generator, <a href= “http://parts.igem.org/Part:BBa_K1379007”> BBa_K1379007 </a></b>: σ<sup>x</sup>-generator was assembled with P<sub>helicase</sub>-GFP generator. Following the assemblage of both constructs, GFP was successfully expressed.
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- | </ul>
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- | </p>
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| | | |
- | </div>
| + | <div class = "catalog_table_area"> |
- | </td>
| + | <table class= "catalog_table"> |
- | </tr>
| + | <thead> |
- | </table>
| + | <tr> |
- | </div>
| + | <th scope="col">Name</th> |
- |
| + | <th scope="col">Type</th> |
- | <table class= "site_map_table" align= "center">
| + | <th scope="col">Description</th> |
- | <tr>
| + | <th scope="col">Length</th> |
- | <td class= 'site_map_column'><h4><a href="https://2014.igem.org/Team:Hong_Kong_HKUST">Home</a></h4>
| + | </tr> |
- | <ul class= 'site_list'>
| + | </thead> |
- | <li class='site_link'><a href="">Pneumosensor</a></li>
| + | <tbody> |
- | <li class='site_link'><a href="">Riboregulator</a></li>
| + | <tr> |
- | <li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice">Human Practice</a></li>
| + | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379004">BBa_K1379004</a></td> |
- | <li class='site_link'><a href="">Team</a></li>
| + | <td>Coding</td> |
- | <li class='site_link'><a href="">WetLab</a></li>
| + | <td>σ<sup>x</sup></td> |
- | <li class='site_link'><a href="">Achievements</a></li>
| + | <td>513</td> |
- | </ul>
| + | </tr> |
- |
| + | |
- | </td>
| + | <tr> |
- | <td class= 'site_map_column'><h4><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor">Pneumosensor</a></h4>
| + | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379006">BBa_K1379006</a></td> |
- | <ul class= 'site_list'>
| + | <td>Generator</td> |
- | <li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/module_one">Sensing</a></li>
| + | <td>σ<sup>x</sup> Generator</td> |
- | <li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/module_two">Expressing</a></li>
| + | <td>711</td> |
- | <li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/parts">Parts</a></li>
| + | </tr> |
- | <li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/data">Data</a></li>
| + | |
- | <li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/characterization">Characterization</a></li>
| + | |
- | <li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/results">Results</a></li>
| + | <tr> |
- | <li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/pneumosensor/future_work">Future Work</a></li>
| + | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379002">BBa_K1379002</a></td> |
- | </ul>
| + | <td>Measurement</td> |
- | </td>
| + | <td>P<sub>celA</sub>-E0240 (Measurement Kit for P<sub>celA</sub>)</td> |
- | <td class= 'site_map_column'><h4><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator">Riboregulator</a></h4>
| + | <td>984</td> |
- | <ul class= 'site_list'>
| + | </tr> |
- | <li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/CR_TA_Feature_Page">Feature Page</a></li> | + | |
- | <li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/RNA_devices_catalog">Catalog Page</a></li> | + | <tr> |
- | <li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/parts">Parts</a></li> | + | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379003">BBa_K1379003</a></td> |
- | <li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/data">Data</a></li>
| + | <td>Measurement</td> |
- | <li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/characterization">Characterization</a></li>
| + | <td>P<sub>comFA</sub>-E0240 (Measurement Kit for P<sub>comFA</sub>)</td> |
- | <li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/results">Results</a></li>
| + | <td>1044</td> |
- | <li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/future_work">Future Work</a></li>
| + | </tr> |
- | </ul>
| + | |
- | </td>
| + | |
- | <td class= 'site_map_column'><h4><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice">Human Practice</a></h4>
| + | |
- | <ul class= 'site_list'>
| + | |
- | <li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/start-up_kit">Start-up Kit</a> | + | |
- | <ul>
| + | |
- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/start-up_kit/handbook" >--Handbook</a></li>
| + | |
- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/start-up_kit/report" >--Report</a></li>
| + | |
- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/start-up_kit/database" >--Database</a></li>
| + | |
- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/start-up_kit/interview" >--Interview</a></li>
| + | |
- | </ul>
| + | |
- | </li> | + | |
- | <li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/outreach">Outreach</a> | + | |
- | <ul>
| + | |
- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/outreach/Workshop" >--Workshop</a></li>
| + | |
- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/outreach/talks" >--Talk</a></li>
| + | |
- | <li><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/outreach/isf_academy" >--ISF Academy</a></li>
| + | |
- | </ul>
| + | |
- | </li>
| + | |
- | <li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/human_practice/safety_and_ethics">Safety and Ethics</a></li>
| + | |
- | </ul>
| + | |
- | </td>
| + | |
- | <td class= 'site_map_column'><h4><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/team">Team</a></h4>
| + | |
- | <ul class= 'site_list'>
| + | |
- | <li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/team/members">Members</a></li> | + | |
- | <li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/team/advisers">Advisers</a></li> | + | |
- | <li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/team/instructors">Instructors</a></li>
| + | |
- | <li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/team/attribution">Attributions</a></li>
| + | |
- | <li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/team/acknowledgement">Acknowledgement</a></li>
| + | |
- | <li class='site_link'><a href="https://igem.org/Team.cgi?year=2014">Official Team Profile</a></li>
| + | |
- | </ul>
| + | |
- | </td>
| + | |
- | <td class= 'site_map_column'><h4><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/wetlab">WetLab</a></h4>
| + | |
- | <ul class= 'site_list'>
| + | |
- | <li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/wetlab/notebook">Notebook</a></li>
| + | |
- | <li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/wetlab/protocols">Protocols</a></li>
| + | |
- | <li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/wetlab/safety">Safety</a></li>
| + | |
- | </ul>
| + | |
- | </td>
| + | |
- | <td class= 'site_map_column'><h4><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/Achievements">Achievement</a></h4>
| + | |
- | <ul class= 'site_list'>
| + | |
- | <li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/acheivement/medal_requirement">Medal Requirements</a></li> | + | |
- | <li class='site_link'><a href="https://2014.igem.org/Team:Hong_Kong_HKUST/acheivement/deliverable">Deliverable</a></li>
| + | |
- | </ul>
| + | |
- | </td>
| + | |
| | | |
- | </tr>
| + | <tr> |
- |
| + | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379045">BBa_K1379045</a></td> |
- | </table>
| + | <td>Composite</td> |
| + | <td>σ<sup>x</sup> + B0015</td> |
| + | <td>650</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379000">BBa_K1379000</a></td> |
| + | <td>Regulatory</td> |
| + | <td>P<sub>celA</sub> 100 basepairs combox promoter</td> |
| + | <td>100</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379001">BBa_K1379001</a></td> |
| + | <td>Regulatory</sub></td> |
| + | <td>P<sub>comFA</sub> 160 base pairs combox promoter</td> |
| + | <td>160</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379005">BBa_K1379005</a></td> |
| + | <td>Measurement</td> |
| + | <td>σ<sup>x</sup> + P<sub>celA</sub>-E0240</td> |
| + | <td>1703</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379007">BBa_K1379007</a></td> |
| + | <td>Measurement</td> |
| + | <td>σ<sup>x</sup> + P<sub>comFA</sub>-E0240</td> |
| + | <td>1763</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379051">BBa_K1379051</a></td> |
| + | <td>Coding</td> |
| + | <td><i>comE</i></td> |
| + | <td>753</td> |
| + | </tr> |
| + | |
| + | </tbody> |
| + | </table> |
| + | |
| + | </div> |
| + | </div> |
| </div> | | </div> |
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| </body> | | </body> |
| </html> | | </html> |
| + | }} |