"
Team:TU Darmstadt/Notebook/Materials/Stock Solution
From 2014.igem.org
(Difference between revisions)
| Line 15: | Line 15: | ||
<div id="wikicontent" class="grid_19"> | <div id="wikicontent" class="grid_19"> | ||
| - | <!--TYPO3SEARCH_begin--><div id="c77" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">Stock Solutions</h1></div><div><p><b>Antibiotics</b></p></div><div></div><div><p>Ampicillin</p></div><div></div><div><p>1.Mix</p></div><div class="indent"><p>- 4 g ampicillin (100 mg/mL)</p></div><div class="indent"><p>- ad. 40 mL ddH<sub>2</sub>O</p></div><div><p>2. sterile filtration</p></div><div><p>3. aliquot in 1 mL stocks and store at -20°C</p></div><div><p>4. use 1 µL per 1 mL medium</p></div><div></div><div><p>Chloramphenicol</p></div><div></div><div><p>1.Mix</p></div><div class="indent"><p>- 1 g chloramphenicol</p></div><div class="indent"><p>- ad. 40 mL ethanol</p></div><div><p>2. aliquot in 1mL stocks and store at -20°C</p></div><div><p>3. use 1µL per 1mL medium</p></div><div></div><div><p>Kanamycin</p></div><div></div><div><p>1.Mix</p></div><div class="indent"><p>- 3 g kanamycin (75 mg/mL)</p></div><div class="indent"><p>- ad. 40 mL ddH<sub>2</sub>O</p></div><p>2.sterile filtration</p><div><p>3.aliquot in 1 mL stocks and store at -20°C</p></div><div><p>4. use 1µL per 1mL medium</p></div><div></div><div></div><div><p><b>Induction chemicals</b></p></div><div></div><div><p>IPTG (Isopropyl-beta-D-thiogalactopyranoside)</p></div><div></div><div><p>1. Dissolve 238 mg IPTG in 10 mL water</p></div><div><p>2. store in 1mL aliquots at -20°C</p></div><div></div><div><p> </p> | + | <!--TYPO3SEARCH_begin--><div id="c77" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">Stock Solutions</h1></div><div><p><b>Antibiotics</b> </p></div><div></div><div><p>Ampicillin</p></div><div></div><div><p>1.Mix</p></div><div class="indent"><p>- 4 g ampicillin (100 mg/mL)</p></div><div class="indent"><p>- ad. 40 mL ddH<sub>2</sub>O</p></div><div><p>2. sterile filtration</p></div><div><p>3. aliquot in 1 mL stocks and store at -20°C</p></div><div><p>4. use 1 µL per 1 mL medium</p></div><div></div><div><p>Chloramphenicol</p></div><div></div><div><p>1.Mix</p></div><div class="indent"><p>- 1 g chloramphenicol</p></div><div class="indent"><p>- ad. 40 mL ethanol</p></div><div><p>2. aliquot in 1mL stocks and store at -20°C</p></div><div><p>3. use 1µL per 1mL medium</p></div><div></div><div><p>Kanamycin</p></div><div></div><div><p>1.Mix</p></div><div class="indent"><p>- 3 g kanamycin (75 mg/mL)</p></div><div class="indent"><p>- ad. 40 mL ddH<sub>2</sub>O</p></div><p>2.sterile filtration</p><div><p>3.aliquot in 1 mL stocks and store at -20°C</p></div><div><p>4. use 1µL per 1mL medium</p></div><div></div><div></div><div><p><b>Induction chemicals</b></p></div><div></div><div><p>IPTG (Isopropyl-beta-D-thiogalactopyranoside)</p></div><div></div><div><p>1. Dissolve 238 mg IPTG in 10 mL water</p></div><div><p>2. store in 1mL aliquots at -20°C</p></div><div></div><div><p> </p> |
<p><b>Loading dye</b></p></div><div></div><div><p>- <a href="https://www.neb.com/products/b7024-gel-loading-dye-purple-6x" title="Link to neb.com" target="_blank" class="external-link-new-window">Gel Loading Dye Purple (6x)</a> </p></div></div><!--TYPO3SEARCH_end--> | <p><b>Loading dye</b></p></div><div></div><div><p>- <a href="https://www.neb.com/products/b7024-gel-loading-dye-purple-6x" title="Link to neb.com" target="_blank" class="external-link-new-window">Gel Loading Dye Purple (6x)</a> </p></div></div><!--TYPO3SEARCH_end--> | ||
</div> | </div> | ||
</html> | </html> | ||
Revision as of 17:31, 17 October 2014
Stock Solutions
Antibiotics
Ampicillin
1.Mix
- 4 g ampicillin (100 mg/mL)
- ad. 40 mL ddH2O
2. sterile filtration
3. aliquot in 1 mL stocks and store at -20°C
4. use 1 µL per 1 mL medium
Chloramphenicol
1.Mix
- 1 g chloramphenicol
- ad. 40 mL ethanol
2. aliquot in 1mL stocks and store at -20°C
3. use 1µL per 1mL medium
Kanamycin
1.Mix
- 3 g kanamycin (75 mg/mL)
- ad. 40 mL ddH2O
2.sterile filtration
3.aliquot in 1 mL stocks and store at -20°C
4. use 1µL per 1mL medium
Induction chemicals
IPTG (Isopropyl-beta-D-thiogalactopyranoside)
1. Dissolve 238 mg IPTG in 10 mL water
2. store in 1mL aliquots at -20°C
Loading dye
