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Team:TU Darmstadt/Notebook/Materials/Buffers
From 2014.igem.org
(Difference between revisions)
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<p>- add ddH<sub>2</sub>O up to 500 mL of volume | <p>- add ddH<sub>2</sub>O up to 500 mL of volume | ||
</p> | </p> | ||
| - | + | </p> | |
<p><b>1x PBS</b> | <p><b>1x PBS</b> | ||
</p> | </p> | ||
| Line 38: | Line 38: | ||
<p>- adjust to pH 7.4 | <p>- adjust to pH 7.4 | ||
</p> | </p> | ||
| - | + | </p> | |
<p><b>TE buffer</b> | <p><b>TE buffer</b> | ||
</p> | </p> | ||
| Line 45: | Line 45: | ||
<p>- 1 mM EDTA (Ethylenediaminetetraacetic acid) | <p>- 1 mM EDTA (Ethylenediaminetetraacetic acid) | ||
</p> | </p> | ||
| - | + | </p> | |
<p><b>50x TAE</b> | <p><b>50x TAE</b> | ||
</p> | </p> | ||
Latest revision as of 17:22, 17 October 2014
Buffers
Tris (1M, pH 7,5)
- 60,5g Tris base
- adjust pH to 7,5 using 5M HCl
- add ddH2O up to 500 mL of volume
1x PBS
- 8.18 g NaCl (140 mM)
- 0.2 g KCl (2.7 mM)
- 1.77 g Na2HPO4 (10 mM)
- 0.24 g KH2PO4 (1.8 mM)
- add ddH2O up to 1 L of volume
- adjust to pH 7.4
TE buffer
- 10 mM Tris, bring to pH 8.0 with HCl
- 1 mM EDTA (Ethylenediaminetetraacetic acid)
50x TAE
- 242 g Tris base in water
- add 57.1 mL glacial acetic acid
- 100 mL of 500 mM EDTA (pH 8.0) solution
- add ddH2O up to 1 L of volume
