Team:TU Darmstadt/Notebook/Materials/Buffers

From 2014.igem.org

(Difference between revisions)
(Created page with "{{:Team:TU_Darmstadt/Template}} <html> <div id="contentWrap" class="container_24"> <div id="breadcrumbs" class="grid_24"> <p>Sie sind hier:  <a href="http://20...")
 
(One intermediate revision not shown)
Line 4: Line 4:
<html>
<html>
-
<div id="contentWrap" class="container_24">
+
 
-
<div id="breadcrumbs" class="grid_24">
+
-
<p>Sie sind hier:&nbsp; <a href="https://2014.igem.org/Team:TU_Darmstadt/" >wiki</a> &rsaquo;&nbsp;<a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook" >Notebook</a> &rsaquo;&nbsp;<span class="current"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials" >Materials </a> &rsaquo;&nbsp;<span class="current"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/Buffers"> Buffers </a></span></p>
+
-
</div>
+
<div id="leftNavi" class="grid_5">
<div id="leftNavi" class="grid_5">
<nav>
<nav>
-
<ul class="menu"><li class="first"><a href="https://2014.igem.org/Team:TU_Darmstadt/" >Home</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Project" >Project</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results" >Results</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/PolicyandPractices" >Policy & Practices</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Achievements" >Achievements</a></li><li class="active"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook" >Notebook</a><ul class="menu2"><li class="first"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal" >Labjournal</a></li><li class="active"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials" >Materials</a><ul class="menu3"><li class="first"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/Strains" >Strains</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Materials/Media" >Media</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/Enzymes" >Enzymes</a></li><li class="active"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/Buffers" >Buffers</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Materials/Stock_Solution" >Stock Solution</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/Primers" >Primers</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/Equipment" >Equipment</a></li><li class="last"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/PCR_buffers_and_mastermixtures" >PCR buffers and mastermixtures</a></li></ul></li><li class="last"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods" >Methods</a></li></ul></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Team" >Team</a></li><li class="last"><a href="https://2014.igem.org/Team:TU_Darmstadt/Gallery" >Gallery</a></li></ul>
+
<ul class="menu"><li class="first"><a href="https://2014.igem.org/Team:TU_Darmstadt" >Home</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Project" >Project</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results" >Results</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/PolicyandPractices" >Policy & Practices</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Achievements" >Achievements</a></li><li class="active"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook" >Notebook</a><ul class="menu2"><li class="first"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal" >Labjournal</a></li><li class="active"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials" >Materials</a><ul class="menu3"><li class="first"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/Strains" >Strains</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Materials/Media" >Media</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/Enzymes" >Enzymes</a></li><li class="active"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/Buffers" >Buffers</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Materials/Stock_Solution" >Stock Solution</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/Primers" >Primers</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/Equipment" >Equipment</a></li><li class="last"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/PCR_buffers_and_mastermixtures" >PCR buffers and mastermixtures</a></li></ul></li><li class="last"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods" >Methods</a></li></ul></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Team" >Team</a></li><li class="last"><a href="https://2014.igem.org/Team:TU_Darmstadt/Gallery" >Gallery</a></li></ul>
</nav>
</nav>
</div>
</div>
Line 26: Line 23:
<p>- add ddH<sub>2</sub>O up to 500 mL of volume
<p>- add ddH<sub>2</sub>O up to 500 mL of volume
</p>
</p>
-
<p>&nbsp;</p>
+
</p>
<p><b>1x PBS</b>
<p><b>1x PBS</b>
</p>
</p>
Line 41: Line 38:
<p>- adjust to pH 7.4
<p>- adjust to pH 7.4
</p>
</p>
-
<p>&nbsp;</p>
+
</p>
<p><b>TE buffer</b>
<p><b>TE buffer</b>
</p>
</p>
Line 48: Line 45:
<p>- 1 mM EDTA (Ethylenediaminetetraacetic acid)
<p>- 1 mM EDTA (Ethylenediaminetetraacetic acid)
</p>
</p>
-
<p>&nbsp;</p>
+
</p>
<p><b>50x TAE</b>
<p><b>50x TAE</b>
</p>
</p>

Latest revision as of 17:22, 17 October 2014

Home


Buffers

Tris (1M, pH 7,5)

- 60,5g Tris base

- adjust pH to 7,5 using 5M HCl

- add ddH2O up to 500 mL of volume

1x PBS

- 8.18 g NaCl (140 mM)

- 0.2 g KCl (2.7 mM)

- 1.77 g Na2HPO4 (10 mM)

- 0.24 g KH2PO4 (1.8 mM)

- add ddH2O up to 1 L of volume

- adjust to pH 7.4

TE buffer

- 10 mM Tris, bring to pH 8.0 with HCl

- 1 mM EDTA (Ethylenediaminetetraacetic acid)

50x TAE

- 242 g Tris base in water

- add 57.1 mL glacial acetic acid

- 100 mL of 500 mM EDTA (pH 8.0) solution

- add ddH2O up to 1 L of volume