Team:TU Darmstadt/Materials/Media

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<!--TYPO3SEARCH_begin--><div id="c74" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">Media</h1></div><div><header><p><b>1.7-DYT (recipe for 500 mL)</b></p></header></div><div></div><div><p>- 2.5 g NaCl
<!--TYPO3SEARCH_begin--><div id="c74" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">Media</h1></div><div><header><p><b>1.7-DYT (recipe for 500 mL)</b></p></header></div><div></div><div><p>- 2.5 g NaCl
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<p>- 8.0 g tryptone</p></div><div><p>- 5.0 g yeast extract</p></div><div><p>- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)</p></div><div></div><div><p><b>LB (recipe for 1000 mL)</b></p></div><div></div><div><p>- 10 g tryptone</p></div><div><p>- 5 g yeast extract</p></div><div><p>- 10 g NaCl</p></div><div><p>- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)</p></div><div><p>For preparing plates add 15 g agar.</p></div><div></div><div><p><b>SOB (recipe for 1000 mL)</b></p></div><div></div><div><p>- 0.186 g KCl</p></div><div><p>- 2.4 g MgSO4</p></div><div><p>- 0.584 g NaCl</p></div><div><p>- 20 g tryptone</p></div><div><p>- 5 g yeast extract</p></div><div><p>- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)</p></div><div></div><div><p>&nbsp;</p>
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<p>- 8.0 g tryptone</p></div><div><p>- 5.0 g yeast extract</p></div><div><p>- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)</p></div><div></div><div><p><b>LB (recipe for 1000 mL)</b></p></div><div></div><div><p>- 10 g tryptone</p></div><div><p>- 5 g yeast extract</p></div><div><p>- 10 g NaCl</p></div><div><p>- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)</p></div><div><p>- For preparing plates add 15 g agar.</p></div><div></div><div><p><b>SOB (recipe for 1000 mL)</b></p></div><div></div><div><p>- 0.186 g KCl</p></div><div><p>- 2.4 g MgSO4</p></div><div><p>- 0.584 g NaCl</p></div><div><p>- 20 g tryptone</p></div><div><p>- 5 g yeast extract</p></div><div><p>- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)</p></div><div></div><div><p></p>
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<p><b>SOC (recipe for 1000 mL)</b></p></div><div></div><div><p>- 980 mL SOB</p></div><div><p>- 20 mL 1M Glucose</p></div><div></div><div></div><div><p>&nbsp;</p>
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<p><b>SOC (recipe for 1000 mL)</b></p></div><div></div><div><p>- 980 mL SOB</p></div><div><p>- 20 mL 1M Glucose</p></div><div></div><div></div><div></p>
<p><b>Autoclave after mixing.</b></p></div></div><!--TYPO3SEARCH_end-->
<p><b>Autoclave after mixing.</b></p></div></div><!--TYPO3SEARCH_end-->
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Latest revision as of 17:20, 17 October 2014

Home


Media

1.7-DYT (recipe for 500 mL)

- 2.5 g NaCl

- 8.0 g tryptone

- 5.0 g yeast extract

- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)

LB (recipe for 1000 mL)

- 10 g tryptone

- 5 g yeast extract

- 10 g NaCl

- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)

- For preparing plates add 15 g agar.

SOB (recipe for 1000 mL)

- 0.186 g KCl

- 2.4 g MgSO4

- 0.584 g NaCl

- 20 g tryptone

- 5 g yeast extract

- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)

SOC (recipe for 1000 mL)

- 980 mL SOB

- 20 mL 1M Glucose

Autoclave after mixing.

Retrieved from "http://2014.igem.org/Team:TU_Darmstadt/Materials/Media"