Team:Caltech/Project/Methods and Methods
From 2014.igem.org
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<li> Add 100 μL of SOC medium, then incubate in a 37°C shaker for 30-90 minutes to recover </li> | <li> Add 100 μL of SOC medium, then incubate in a 37°C shaker for 30-90 minutes to recover </li> | ||
<li> Plate with glass beads </li> | <li> Plate with glass beads </li> | ||
+ | </ul> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr><td bgColor="#777777" colspan="2" height="1px"></td></tr> | ||
+ | |||
+ | <tr> | ||
+ | <td width="25%" valign="top"> | ||
+ | <h4>Liquid Culture Inoculation</h4> | ||
+ | </td> | ||
+ | <td valign="top"> | ||
+ | <ul><li> Add 5 μL antibiotic to 5 mL LB media </li> | ||
+ | <li> Pick colony from solid culture with pipette tip and mix into liquid </li> | ||
+ | <li> Incubate in 37°C shaker overnight</li> | ||
</ul> | </ul> | ||
</td> | </td> |
Revision as of 23:38, 14 July 2014
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Overall Project Summary
Project Details Materials and Methods The Experiments Results Data Analysis Conclusions References |
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