Team:Evry/Interlab Study/09-17-2014

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<i>PSB1C3+E0240+J23100</i>: <br>
<i>PSB1C3+E0240+J23100</i>: <br>
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h-16h).<br>
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h-16h).<br>
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<FONT color="red">OVERFLOW</FONT> <br>
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<i>PSB1C3+E0240+J23101</i>: <br>
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Measure of the cells' growth and fluorecence on TECAN infiniteM200.<br>
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<FONT color="red">TECAN FAILED</FONT> <br>
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<i>PSB1C3+E0240+J23103</i>: <br>
<i>PSB1C3+E0240+J23103</i>: <br>
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (16h30-23h30).<br>
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (16h30-23h30).<br>
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<img src="" alt="PLAN DE PLAQUE" />
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<img src="https://static.igem.org/mediawiki/2014/6/65/PlaqueLeft.png" alt="PLAN DE PLAQUE" />
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<i>PSB1C3+E0240+J23115</i>: <br>
<i>PSB1C3+E0240+J23115</i>: <br>
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (12h-19h).<br>
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (12h-19h).<br>
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<img src="" alt="PLAN DE PLAQUE" />
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<img src="https://static.igem.org/mediawiki/2014/6/62/J23114.26.09.png" alt="PLAN DE PLAQUE" />
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PSB1C3+E0240+J23104, PSB1C3+E0240+J23112, PSB1C3+E0240+J23113:
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PCR colony of 6 colonies for each construction following protocol table 1 and 2.<br>
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Migration of the PCR products<br>
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PCR purification of clones with the good construction <br>
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    <center>GEL : 1% agarose gel. PCR product of 1 colony of the PSB1C3+E0240+J23104, PSB1C3+E0240+J23112 and PSB1C3+E0240+J23113 constructions. </center>
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A pre-culture was made for each colony to make a glycerol stock.<br>
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Latest revision as of 16:57, 17 October 2014

Picture

Construction n°1: PSB1C3 with I20260




Construction n°2: PSB1C3 with J23101-E1010




Construction n°3: PSB1C3 with K823012-E1010


Pre-culture from glycerol stock in LB+Cam (1:1000). Incubation 16h-18h at 37°C.

Other constructions of the Anderson library of constitutive promoters


PSB1C3+E0240+J23100:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h-16h).
OVERFLOW

PSB1C3+E0240+J23101:
Measure of the cells' growth and fluorecence on TECAN infiniteM200.
TECAN FAILED

PSB1C3+E0240+J23103:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (16h30-23h30).
PLAN DE PLAQUE

PSB1C3+E0240+J23105:
pre-culture of the colony 5 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation overnight at 37°C.

PSB1C3+E0240+J23107:
pre-culture of the colony 5 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation overnight at 37°C.

PSB1C3+E0240+J23110:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

PSB1C3+E0240+J23115:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (12h-19h).
PLAN DE PLAQUE

PSB1C3+E0240+J23104, PSB1C3+E0240+J23112, PSB1C3+E0240+J23113: PCR colony of 6 colonies for each construction following protocol table 1 and 2.
Migration of the PCR products
PCR purification of clones with the good construction
IMAGE
Symbol
GEL : 1% agarose gel. PCR product of 1 colony of the PSB1C3+E0240+J23104, PSB1C3+E0240+J23112 and PSB1C3+E0240+J23113 constructions.
A pre-culture was made for each colony to make a glycerol stock.

Sep 17

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