Team:TU Darmstadt/Notebook/Materials/Media

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<p>Sie sind hier:&nbsp; <a href="https://2014.igem.org/Team:TU_Darmstadt/" >wiki</a> &rsaquo;&nbsp;<a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook" >Notebook</a> &rsaquo;&nbsp;<span class="current"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials" >Materials </a> &rsaquo;&nbsp;<span class="current"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/Media" >Media </a></span></p>
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Revision as of 16:54, 17 October 2014

Home


Media

1.7-DYT (recipe for 500 mL)

- 2.5 g NaCl

- 8.0 g tryptone

- 5.0 g yeast extract

- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)

LB (recipe for 1000 mL)

- 10 g tryptone

- 5 g yeast extract

- 10 g NaCl

- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)

For preparing plates add 15 g agar.

SOB (recipe for 1000 mL)

- 0.186 g KCl

- 2.4 g MgSO4

- 0.584 g NaCl

- 20 g tryptone

- 5 g yeast extract

- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)

 

SOC (recipe for 1000 mL)

- 980 mL SOB

- 20 mL 1M Glucose

 

Autoclave after mixing.

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