"
Team:Caltech/Project/Methods and Methods
From 2014.igem.org
(Difference between revisions)
| Line 98: | Line 98: | ||
</td> | </td> | ||
<td valign="top"> | <td valign="top"> | ||
| - | <ul><li> | + | For each 25 uL reaction mixture: |
| - | <li></li> | + | <ul><li>12.5 uL Phusion Mastermix</li> |
| + | <li>2.5 uL primer mix (10 uM of forward and reverse primer)</li> | ||
| + | <li>1 uL DNA template </li> | ||
| + | <li>0.75 uL DMSO (optional) </li> | ||
| + | <li>Fill to 25 uL with MilliQ water </li> | ||
</ul> | </ul> | ||
</td> | </td> | ||
Revision as of 20:35, 14 July 2014
 | |||||||||||||||||||
| |||||||||||||||||||
| | |||||||||||||||||||
|
Overall Project Summary
Project Details Materials and Methods The Experiments Results Data Analysis Conclusions References |
|
||||||||||||||||||
