Team:Caltech/Project/Methods and Methods
From 2014.igem.org
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- | blah blah | + | <h4>PCR</h4> |
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+ | <ul><li>blah blah</li> | ||
+ | <li>blah blah</li> | ||
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+ | <h4>Wednesday, 6/18/14</h4> | ||
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+ | <ul><li>Continue to discuss project and clean up lab space</li> | ||
+ | <li>Designed PCR primers for combinatorial promoters subproject</li> | ||
+ | </ul> | ||
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+ | <h4>Thursday, 6/19/14</h4> | ||
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+ | <ul><li>Formulated plasmid design (pAA001) for the response regulation subproject</li> | ||
+ | <li>PCR run on plasmid backbone and sfGFP to create constructs with the proper overhangs for Gibson assembly</li> | ||
+ | <li>Gel electrophoresis for confirmation of PCR products' length</li> | ||
+ | </ul> | ||
+ | <hr> | ||
+ | <ul><li>With the exception of the lacI gene, PCR products were confirmed via gel electrophoresis</li><ul> | ||
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+ | <h4>Friday, 6/20/14</h4> | ||
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+ | <ul><li>Purification of PCR products created yesterday</li> | ||
+ | <li>Gibson assembly of purified products</li> | ||
+ | <li>Transformation of Gibson-assembled plasmids into JM109 E. coli</li> | ||
+ | <li>Meeting with Prof. Murray to discuss give an update on the project</li> | ||
+ | </ul> | ||
+ | <hr> | ||
+ | <ul><li>Colonies were found and picked after transformation and incubation.</li></ul> | ||
+ | </td> | ||
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+ | </table> | ||
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Revision as of 18:40, 14 July 2014
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Overall Project Summary
Project Details Materials and Methods The Experiments Results Data Analysis Conclusions References |
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