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Team:Cambridge-JIC/Guide/Materials/Checklist
From 2014.igem.org
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=Checklist= | =Checklist= | ||
| - | This checklist contains | + | This checklist contains the minimum you will need for setting up transformation. |
==DNA== | ==DNA== | ||
| - | - A [[Team:Cambridge-JIC/Guide/Constructs/AgrobacteriumVectors| helper plasmid]] for [[Team:Cambridge-JIC/Guide/Transformation|Transformation] | + | - A [[Team:Cambridge-JIC/Guide/Constructs/AgrobacteriumVectors| helper plasmid]] for [[Team:Cambridge-JIC/Guide/Transformation|Transformation]] |
- Your [[Team:Cambridge-JIC/Guide/Constructs|T-DNA plasmid]] for transformation | - Your [[Team:Cambridge-JIC/Guide/Constructs|T-DNA plasmid]] for transformation | ||
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==[[Team:Cambridge-JIC/Guide/Materials/Strains |Bacteria]]== | ==[[Team:Cambridge-JIC/Guide/Materials/Strains |Bacteria]]== | ||
- Competent E-Coli (chemically/electro) | - Competent E-Coli (chemically/electro) | ||
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OR | OR | ||
| - | - Male and female Marchantia polymorpha [from the garden] | + | - Male and female Marchantia polymorpha [from the garden], for [[Team:Cambridge-JIC/Guide/Care/SexualReproduction|growing spores]] |
==Miscellaneous== | ==Miscellaneous== | ||
- [[Team:Cambridge-JIC/Guide/Materials/Media|Gamborg's B5 salt mix]] | - [[Team:Cambridge-JIC/Guide/Materials/Media|Gamborg's B5 salt mix]] | ||
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==Equipment== | ==Equipment== | ||
- Ventilated cupboard/room/box under full illumination [larger space = more transformations] | - Ventilated cupboard/room/box under full illumination [larger space = more transformations] | ||
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Latest revision as of 15:29, 17 October 2014
Contents |
Checklist
This checklist contains the minimum you will need for setting up transformation.
DNA
- A helper plasmid for Transformation
- Your T-DNA plasmid for transformation
Bacteria
- Competent E-Coli (chemically/electro)
- Electrocompetent agrobacteria
Marchantia
OR
- Male and female Marchantia polymorpha [from the garden], for growing spores
Miscellaneous
- LB/SOC for bacterial transformation and growth
- 100mM Acetosyringone
Equipment
- Ventilated cupboard/room/box under full illumination [larger space = more transformations]
