Team:Tuebingen/Notebook/Protocols/OligoAnnealing

From 2014.igem.org

(Difference between revisions)
Line 10: Line 10:
<div id="TueContent">
<div id="TueContent">
-
<h1>Oligo Annealing</h1>
+
<h3>Oligo Annealing</h3>
<h4>Reagents</h4>
<h4>Reagents</h4>

Revision as of 15:23, 17 October 2014


Oligo Annealing

Reagents

96 µL TE-buffer + 56 mM NaCl
2 µL Primer forward
2 µL Primer reverse

 

Procedure

  1. Dilute the primers in buffer.
  2. Heat the solution to 98 °C and decrease temperature slowly to 25 °C.
  3. Use for ligation.

Retrieved from "http://2014.igem.org/Team:Tuebingen/Notebook/Protocols/OligoAnnealing"