Team:Hong Kong HKUST/wetlab/protocols

From 2014.igem.org

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<h3>Protocols</h3>
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<h4> Mutagenesis</h4>
 
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<h4> Western Blot </h4>
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<h4> <b>Dephosphorylation</b> </h4>
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<h4> LB agar and LB plates </h4>
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<h4> <b>Ethanol Precipitation</b> </h4>
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<h4> Ligation </h4>
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<h4> <b>Gibson Assembly</b> </h4>
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<h4> Ethanol Precipitation</h4>
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<h4> <b>Ligation</b> </h4>
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<a href="https://static.igem.org/mediawiki/2014/2/27/Pbad.pdf">
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<h4> Pbad Protocols </h4>
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<h4> <b>Liquid Mediums and Agarose Plates</b> </h4>
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<a href="https://static.igem.org/mediawiki/2014/8/8b/M9_Minimal_medium_protocol.pdf">
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<h4> Gibson Assembly </h4>
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<h4> <b>M9 Minimal Medium</b> </h4>
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<a href="https://static.igem.org/mediawiki/2014/2/2a/Mutagenesis_protocol_1_%28multiple_sites%29.pdf">
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<h4> Polymerase Chain Reaction (PCR)</h4>
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<h4> <b>Mutagenesis 1 <br>(Multiple Sites)</b></h4>
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<h4> <b>Mutagenesis 2 <br>(Single Site)</b> </h4>
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<a href="https://static.igem.org/mediawiki/2014/d/d0/Protocol_for_a_routine_Taq_PCR.pdf">
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<h4> <b>PCR (routine <i>Taq</i>)</b></h4>
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<a href="https://static.igem.org/mediawiki/2014/0/03/Protocol_for_Phusion_PCR.pdf">
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<h4> <b>PCR (Phusion)</b> </h4>
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<a href="https://static.igem.org/mediawiki/2014/c/c5/Protocol_for_Vent_PCR.pdf">
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<h4> <b>PCR (Vent)</b> </h4>
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<a href="https://static.igem.org/mediawiki/2014/7/76/RPU_Measurement_Method_.pdf">
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<h4> <b>RPU Measurement</b> </h4>
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Latest revision as of 14:52, 17 October 2014



Protocols

We have a variety of protocols that we followed for working on our project. The step-by-step procedures of each technique can be found here!

Home

Pneumosensor

Riboregulator

Human Practice

Team

WetLab

Achievement

Retrieved from "http://2014.igem.org/Team:Hong_Kong_HKUST/wetlab/protocols"