Team:NCTU Formosa/Notebook/test5

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(Difference between revisions)
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</ul>
</ul>
</div>
</div>
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<div class="calendar-note">
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<div class="calendar-note" style="height:251px;">
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<div  class="single note">none<br></div>
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<div  class="single note">
<div  class="single note">
-
<p>4/11
+
<font color="#FF0000"><span style="font-size:1cm;">None</span></font><br>
-
Transform of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr  (G2)
+
-
Transform of [Plac+PSB1A3] Ar, [mGFP+PSB1A3] Ar  (G3)     
+
-
Transform of [PSB1K3] Kr,[PSB1A3] Ar, [PSB1C3] Cr  (G1)
+
-
<p>
+
-
<p>4/12
+
-
Cultivation of  [PSB1K3] Kr, [PSB1C3] Cr (G2)
+
-
Cultivation of  [PSB1A3] Ar, [PSB1C3] Cr (G1)
+
-
Cultivation of [mGFP] Ar (G3)
+
-
</p>
+
<br></div>
<br></div>
-
<div class="single note">
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<div class="single note">
-
 
+
<font color="#FF0000"><span style="font-size:1cm;">None</span></font><br>
-
<p>4/13
+
-
Mini-prep of [mGFP] Ar, [PSB1K3] Kr(G3)
+
-
Mini-prep of [PSB1K3] Kr, [PSB1C3] Cr (G2)
+
-
Mini-prep of [PSB1A3] Ar, [PSB1C3] Cr (G1)</p>
+
-
 
+
-
<p>4/17
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Transform of [PSB1K3] Kr (G1)</p>
+
-
 
+
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<p>4/18
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Cultivation of [PSB1K3] Kr (G1) </p>
+
-
 
+
-
<p>4/19
+
-
Transform of [Plac+PSB1A3] Ar, [PSB1K3] Kr , [PSB1A3] Ar, [PSB1C3] Cr (G3)
+
-
Mini-prep of [PSB1K3] Kr(G1) </p>
+
-
 
+
-
 
+
<br></div>
<br></div>
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<div class="single note">
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<div  class="single note">
-
<p>4/20
+
-
Transform of [PSB1A3] Ar (G2)
+
-
Electrophoresis of [PSB1K3]Kr, [PSB1C3]Cr plasmid (G2)</p>
+
-
 
+
-
<p>4/21
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Cultivation of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)</p>
+
-
 
+
-
<p>4/22
+
-
Mini-prep of  [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)</p>
+
-
<br></div>
+
-
<div class="single note">
+
<p>
<p>
-
4/28
+
<font color="#FF0000"><span style="font-size:1cm;">8/11</span></font><br></p>
-
Electrophoresis of digested [PSB1K3] Kr(EP), [PSB1C3]  Cr(EP),[PSB1A3] Ar(EP) (G1)
+
-
Cultivation of [PSB1C3] Cr (G1)
+
-
</p>
+
<p>
<p>
-
4/29
+
<font color="yellow">Transformation of</font> NCTU1 (G1)</p>
-
Mini-prep of [PSB1C3] Cr(G1)
+
-
Mini-prep of [PSB1C3]Cr (G2)
+
-
Transform of [B0032]Ar, and [BFP]Cr (G2)
+
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Cultivation of [PSB1K3] Kr, [PSB1A3] Ar (G1)
+
-
Cultivation of [B0032]Ar, and [BFP]Cr (G2)
+
-
</p>
+
<p>
<p>
-
4/30
+
<font color="yellow">Transformation of</font> PBAN (G2)</p>
-
Mini-prep of [PSB1A3] Ar (G1)
+
<p>
-
Mini-prep of [B0032]Ar, and [BFP]Cr (G2)
+
<font color="#FF0000"><span style="font-size:1cm;">8/12</span></font><br></p>
-
Transform of [B0032]Ar, and [BFP]Cr, [T7promoter]ArKr and [T7 polymerase]Cr (G2)
+
<p>
-
Transform of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)
+
<font color="yellow">Cultivation of</font> [PBAN-4]Kr,[PBAN-5]Krand [PBAN-6]Kr E.coli in LB broth (G1)</p>
-
Digestion of [PSBIC3]Cr(EP), [B0032]Ar(ES), [BFP]Cr(XP) mini (G2)
+
<p>
-
Electrophoresis of plasmid and digested prouct of [PSBIC3]Cr(EP), [B0032]Ar(ES), [BFP]Cr(XP) mini (G2)
+
<font color="yellow">Cultivation of </font> [RBS+BFP+J61048]Cr ,[T7+RBS+BFP+J61048]Ar Kr</p>
-
</p>
+
<p>
 +
<font color="yellow">Mini-prep of</font> [P<sub>cons</sub>]Ar</p>
 +
<p>
 +
<font color="yellow">Digestion of</font> [P<sub>cons</sub>]Ar (ES) (G2)</p>
 +
<p>
 +
<font color="#FF0000"><span style="font-size:1cm;">8/13</span></font><br></p>
 +
<p>
 +
<font color="yellow">Mini-prep of</font> [PBAN-4] Kr,[PBAN-5] Krand [PBAN-6] Kr (G1)</p>
 +
<p>
 +
<font color="yellow">Digestion of</font> [PBAN-4]Kr(EP),[PBAN-5]Kr(EP)and [PBAN-6]Kr(EP) (G1)</p>
 +
<p>
 +
<font color="yellow">Electrophoresis of</font> [P<sub>cons</sub>](mini)&(dig ES),digestion of [P<sub>cons</sub>](XP)
 +
</p><p>
 +
<font color="yellow">Ligation of</font> [P<sub>cons</sub>]+[RBS+BFP+J61048] (G2)</p>
 +
<p>
 +
<font color="#FF0000"><span style="font-size:1cm;">8/14</span></font><br></p>
 +
<p>
 +
<font color="yellow">Electrophoresis of</font> plasmid and digestion products of  [PBAN-4] Kr(EP),[PBAN-5] Kr(EP)and [PBAN-6] Kr(EP) (G1)</p>
 +
<p>
 +
<font color="yellow">Purification from electrophoresis of</font> [PBAN]No.4&5&6(G1)</p>
 +
<p>
 +
<font color="yellow">Transformation of</font> the ligation product made yesterday</p>
 +
<p>
 +
<font color="yellow">Electrophoresis of</font> plasmid and digestion products of [PBAN]No.4&5&6,[P<sub>cons</sub>],[P<sub>cons</sub>+RBS+BFP+J61048] (G2)</p>
 +
<p>
 +
<font color="#FF0000"><span style="font-size:1cm;">8/15</span></font><br></p>
 +
<p>
 +
<font color="yellow">Digestion of</font> purification products yesterday:[PBAN]No.4&5&6 (EP) (G1)</p>
 +
<p>
 +
<font color="yellow">Purification from electrophoresis of</font> [PBAN]No.4&5&6</p>
 +
<p>
 +
<font color="yellow">Transformation of</font> [B0034]Ar (G2)</p>
 +
<p>
 +
<font color="#FF0000"><span style="font-size:1cm;">8/16</span></font><br></p>
 +
<p>
 +
<font color="yellow">Electrophoresis of</font> the purification products from electrophoresis,digestion products of[PBAN]No.4&5&6 (EP) (G1)</p>
 +
<p>
 +
<font color="yellow">PCR of</font> [B0034]</p>
 +
<p>
 +
<font color="yellow">Electrophoresis of</font> [PSB1K3](mini)&(dig E),[P<sub>cons</sub>](dig SP),PCR products of B0034</p>
 +
<p>
 +
<font color="yellow">Mini-prep of</font> [B0034]Ar E.coli</p>
 +
<p>
 +
<font color="yellow">Ligation of</font> [P<sub>cons</sub>]+[B0034] +PSB1K3</p>
 +
<p>
 +
<font color="yellow">Digestion of</font> [BFP](ES)</p>
 +
<p>
 +
<font color="yellow">Electrophoresis of </font> plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[P<sub>cons</sub>])</p>
 +
<br></div>
 +
<div  class="single note">
 +
<p>
 +
<font color="#FF0000"><span style="font-size:1cm;">8/17</span></font><br></p>
 +
<p>
 +
<font color="yellow">Electrophoresis of</font> the digestion products of[PBAN]No.4&5&6 (EP) (G1)</p>
 +
<br></div>
 +
<div  class="single note">
 +
<font color="#FF0000"><span style="font-size:1cm;">None</span></font><br>
 +
<br></div>
 +
<div  class="single note">
 +
<font color="#FF0000"><span style="font-size:1cm;">None</span></font><br>
<br></div>
<br></div>
-
 
</div>
</div>
 +
<div class="clear"></div>
<div class="clear"></div>
</div>
</div>

Latest revision as of 14:24, 17 October 2014

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None

None

8/11

Transformation of NCTU1 (G1)

Transformation of PBAN (G2)

8/12

Cultivation of [PBAN-4]Kr,[PBAN-5]Krand [PBAN-6]Kr E.coli in LB broth (G1)

Cultivation of [RBS+BFP+J61048]Cr ,[T7+RBS+BFP+J61048]Ar Kr

Mini-prep of [Pcons]Ar

Digestion of [Pcons]Ar (ES) (G2)

8/13

Mini-prep of [PBAN-4] Kr,[PBAN-5] Krand [PBAN-6] Kr (G1)

Digestion of [PBAN-4]Kr(EP),[PBAN-5]Kr(EP)and [PBAN-6]Kr(EP) (G1)

Electrophoresis of [Pcons](mini)&(dig ES),digestion of [Pcons](XP)

Ligation of [Pcons]+[RBS+BFP+J61048] (G2)

8/14

Electrophoresis of plasmid and digestion products of [PBAN-4] Kr(EP),[PBAN-5] Kr(EP)and [PBAN-6] Kr(EP) (G1)

Purification from electrophoresis of [PBAN]No.4&5&6(G1)

Transformation of the ligation product made yesterday

Electrophoresis of plasmid and digestion products of [PBAN]No.4&5&6,[Pcons],[Pcons+RBS+BFP+J61048] (G2)

8/15

Digestion of purification products yesterday:[PBAN]No.4&5&6 (EP) (G1)

Purification from electrophoresis of [PBAN]No.4&5&6

Transformation of [B0034]Ar (G2)

8/16

Electrophoresis of the purification products from electrophoresis,digestion products of[PBAN]No.4&5&6 (EP) (G1)

PCR of [B0034]

Electrophoresis of [PSB1K3](mini)&(dig E),[Pcons](dig SP),PCR products of B0034

Mini-prep of [B0034]Ar E.coli

Ligation of [Pcons]+[B0034] +PSB1K3

Digestion of [BFP](ES)

Electrophoresis of plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[Pcons])


8/17

Electrophoresis of the digestion products of[PBAN]No.4&5&6 (EP) (G1)


None

None

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