Team:NCTU Formosa/Notebook/test5

From 2014.igem.org

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None

None

8/11

Transformation of NCTU1 (G1)

Transformation of PBAN (G2)

8/12

Cultivation of [PBAN-4]Kr,[PBAN-5]Krand [PBAN-6]Kr E.coli in LB broth (G1)

Cultivation of [RBS+BFP+J61048]Cr ,[T7+RBS+BFP+J61048]Ar Kr

Mini-prep of [Pcons]Ar

Digestion of [Pcons]Ar (ES) (G2)

8/13

Mini-prep of [PBAN-4] Kr,[PBAN-5] Krand [PBAN-6] Kr (G1)

Digestion of [PBAN-4]Kr(EP),[PBAN-5]Kr(EP)and [PBAN-6]Kr(EP) (G1)

Electrophoresis of [Pcons](mini)&(dig ES),digestion of [Pcons](XP)

Ligation of [Pcons]+[RBS+BFP+J61048] (G2)

8/14

Electrophoresis of plasmid and digestion products of [PBAN-4] Kr(EP),[PBAN-5] Kr(EP)and [PBAN-6] Kr(EP) (G1)

Purification from electrophoresis of [PBAN]No.4&5&6(G1)

Transformation of the ligation product made yesterday

Electrophoresis of plasmid and digestion products of [PBAN]No.4&5&6,[Pcons],[Pcons+RBS+BFP+J61048] (G2)

8/15

Digestion of purification products yesterday:[PBAN]No.4&5&6 (EP) (G1)

Purification from electrophoresis of [PBAN]No.4&5&6

Transformation of [B0034]Ar (G2)

8/16

Electrophoresis of the purification products from electrophoresis,digestion products of[PBAN]No.4&5&6 (EP) (G1)

PCR of [B0034]

Electrophoresis of [PSB1K3](mini)&(dig E),[Pcons](dig SP),PCR products of B0034

Mini-prep of [B0034]Ar E.coli

Ligation of [Pcons]+[B0034] +PSB1K3

Digestion of [BFP](ES)

Electrophoresis of plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[Pcons])


8/17

Electrophoresis of the digestion products of[PBAN]No.4&5&6 (EP) (G1)


None

None

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