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Team:UCL/Results/Deg
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<!-- Titles go in a <h1>TITLE GOES HERE</h1> and h1 is this biggest title and h6 is the smallest. all paragraphs go in <p>paragraph goes here</p> tags. Images go in as <img src="url of image here"> and to upload an image go to https://2014.igem.org/Special:Upload. Upload the image then click on the image which takes you to a page with only an image on it. The url of the image is the image you want to use. Use google and ask Lewis and Adam as much as you want--> | <!-- Titles go in a <h1>TITLE GOES HERE</h1> and h1 is this biggest title and h6 is the smallest. all paragraphs go in <p>paragraph goes here</p> tags. Images go in as <img src="url of image here"> and to upload an image go to https://2014.igem.org/Special:Upload. Upload the image then click on the image which takes you to a page with only an image on it. The url of the image is the image you want to use. Use google and ask Lewis and Adam as much as you want--> | ||
| - | DEGRADATION TAB | + | <h2>DEGRADATION TAB</h2> |
In order to determine the compatibility of our <a href="http://parts.igem.org/Part:BBa_K1336003">BBa_K1336003</a> BsDyP Azo-degradation module, we set out to investigate the growth performance of E.coli DH5α containing our BioBrick in different media. We then set out to investigate the functionality of our Azo-degradation module in decolourising several model Azo-dye contaminated waste-waters. | In order to determine the compatibility of our <a href="http://parts.igem.org/Part:BBa_K1336003">BBa_K1336003</a> BsDyP Azo-degradation module, we set out to investigate the growth performance of E.coli DH5α containing our BioBrick in different media. We then set out to investigate the functionality of our Azo-degradation module in decolourising several model Azo-dye contaminated waste-waters. | ||
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PLEASE INSERT: https://2014.igem.org/File:BsDyP_Figure_1.png | PLEASE INSERT: https://2014.igem.org/File:BsDyP_Figure_1.png | ||
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<b>Figure 1 - <a href="http://parts.igem.org/Part:BBa_K1336003">BBa_K1336003</a> BsDyP Azo-degradation module preserves growth performance of E.coli DH5α in LB media. </b> Graph showing that E.coli transformed with the BBa_K1336003 BsDyP Azo-degradation module shows comparable growth the plasmid-free control in LB media. OD measured at 680nm and Time is shown in hours after incubation. Error bars indicate SEM, n=2. | <b>Figure 1 - <a href="http://parts.igem.org/Part:BBa_K1336003">BBa_K1336003</a> BsDyP Azo-degradation module preserves growth performance of E.coli DH5α in LB media. </b> Graph showing that E.coli transformed with the BBa_K1336003 BsDyP Azo-degradation module shows comparable growth the plasmid-free control in LB media. OD measured at 680nm and Time is shown in hours after incubation. Error bars indicate SEM, n=2. | ||
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PLEASE INSERT: https://static.igem.org/mediawiki/2014/7/7f/Bsdyp_figure_2.png | PLEASE INSERT: https://static.igem.org/mediawiki/2014/7/7f/Bsdyp_figure_2.png | ||
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<b>Figure 2 - <a href="http://parts.igem.org/Part:BBa_K1336003">BBa_K1336003</a> BsDyP Azo-degradation module is compatible with Acid Orange 7 (AO7) dye-contaminated waste waters. </b> Graph showing that E.coli transformed with the BBa_K1336003 BsDyP Azo-degradation module is able to grow in LB media contaminated with AO7 dye. Please note that OD measurements are considerably higher in dye-contaminated waters due to the absorbance of the azo-dye. OD measured at 680nm and Time is shown in hours after incubation. Error bars indicate SEM, n=2. | <b>Figure 2 - <a href="http://parts.igem.org/Part:BBa_K1336003">BBa_K1336003</a> BsDyP Azo-degradation module is compatible with Acid Orange 7 (AO7) dye-contaminated waste waters. </b> Graph showing that E.coli transformed with the BBa_K1336003 BsDyP Azo-degradation module is able to grow in LB media contaminated with AO7 dye. Please note that OD measurements are considerably higher in dye-contaminated waters due to the absorbance of the azo-dye. OD measured at 680nm and Time is shown in hours after incubation. Error bars indicate SEM, n=2. | ||
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PLEASE INSERT: https://static.igem.org/mediawiki/2014/d/d6/Bsdyp_figure3.png | PLEASE INSERT: https://static.igem.org/mediawiki/2014/d/d6/Bsdyp_figure3.png | ||
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<b>Figure 3 - <a href="http://parts.igem.org/Part:BBa_K1336003">BBa_K1336003</a> BsDyP Azo-degradation module is compatible with Reactive Black 5 (RB5) dye-contaminated waste waters. </b> Graph showing that E.coli transformed with the BBa_K1336003 BsDyP Azo-degradation module is able to grow in LB media contaminated with RB5 dye. Please note that OD measurements are considerably higher in dye-contaminated waters due to the absorbance of the azo-dye. OD measured at 680nm and Time is shown in hours after incubation. Error bars indicate SEM, n=2. | <b>Figure 3 - <a href="http://parts.igem.org/Part:BBa_K1336003">BBa_K1336003</a> BsDyP Azo-degradation module is compatible with Reactive Black 5 (RB5) dye-contaminated waste waters. </b> Graph showing that E.coli transformed with the BBa_K1336003 BsDyP Azo-degradation module is able to grow in LB media contaminated with RB5 dye. Please note that OD measurements are considerably higher in dye-contaminated waters due to the absorbance of the azo-dye. OD measured at 680nm and Time is shown in hours after incubation. Error bars indicate SEM, n=2. | ||
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In order to determine the capabilities of our <a href="http://parts.igem.org/Part:BBa_K1336003">BBa_K1336003</a> and <a href="http://parts.igem.org/Part:BBa_K1336007">BBa_K1336007</a> BsDyP Azo-degradation module, DANIEL TO FILL OUT! | In order to determine the capabilities of our <a href="http://parts.igem.org/Part:BBa_K1336003">BBa_K1336003</a> and <a href="http://parts.igem.org/Part:BBa_K1336007">BBa_K1336007</a> BsDyP Azo-degradation module, DANIEL TO FILL OUT! | ||
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PLEASE INSERT: https://static.igem.org/mediawiki/2014/d/db/Bsdyp_fig4.png | PLEASE INSERT: https://static.igem.org/mediawiki/2014/d/db/Bsdyp_fig4.png | ||
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<b>Figure 4 - <a href="http://parts.igem.org/Part:BBa_K1336007">BBa_K1336007</a> BsDyP Azo-degradation module is capable of degrading Acid Orange 7 (AO7) dye-contaminated waste waters. </b> Graph showing that in comparison to the plasmid free control, E.coli transformed with the BBa_K1336007 BsDyP Azo-degradation module is able to decolourise AO7 dye contaminated LB media after being induced by 1mM IPTG. Inoculations were grown at 37 degrees and 180rpm for 24 hours and then left stationary for a further 24 hours at room temperature. OD680nm measurements were taken of the supernatant at the end of the 48 hour experiment. Error bars indicate SEM, n=2. | <b>Figure 4 - <a href="http://parts.igem.org/Part:BBa_K1336007">BBa_K1336007</a> BsDyP Azo-degradation module is capable of degrading Acid Orange 7 (AO7) dye-contaminated waste waters. </b> Graph showing that in comparison to the plasmid free control, E.coli transformed with the BBa_K1336007 BsDyP Azo-degradation module is able to decolourise AO7 dye contaminated LB media after being induced by 1mM IPTG. Inoculations were grown at 37 degrees and 180rpm for 24 hours and then left stationary for a further 24 hours at room temperature. OD680nm measurements were taken of the supernatant at the end of the 48 hour experiment. Error bars indicate SEM, n=2. | ||
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PLEASE INSERT: https://static.igem.org/mediawiki/2014/4/47/Bsdyp_fig5.png | PLEASE INSERT: https://static.igem.org/mediawiki/2014/4/47/Bsdyp_fig5.png | ||
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<b>Figure 5 - <a href="http://parts.igem.org/Part:BBa_K1336007">BBa_K1336007</a> BsDyP Azo-degradation module is capable of degrading Reactive Black 5 (RB5) dye-contaminated waste waters. </b> Graph showing that in comparison to the plasmid free control, E.coli transformed with the BBa_K1336007 BsDyP Azo-degradation module is able to decolourise RB5 dye-contaminated LB media after being induced by 1mM IPTG. Inoculations were grown at 37 degrees and 180rpm for 24 hours and then left stationary for a further 24 hours at room temperature. OD680nm measurements were taken of the supernatant at the end of the 48 hour experiment. Error bars indicate SEM, n=2. | <b>Figure 5 - <a href="http://parts.igem.org/Part:BBa_K1336007">BBa_K1336007</a> BsDyP Azo-degradation module is capable of degrading Reactive Black 5 (RB5) dye-contaminated waste waters. </b> Graph showing that in comparison to the plasmid free control, E.coli transformed with the BBa_K1336007 BsDyP Azo-degradation module is able to decolourise RB5 dye-contaminated LB media after being induced by 1mM IPTG. Inoculations were grown at 37 degrees and 180rpm for 24 hours and then left stationary for a further 24 hours at room temperature. OD680nm measurements were taken of the supernatant at the end of the 48 hour experiment. Error bars indicate SEM, n=2. | ||
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LEAVE SOME SPACE - next paragraph | LEAVE SOME SPACE - next paragraph | ||
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In order to widen our Azo-degrading and de-colourising toolbox, we also set out to investigate whether we could incorporate the <a href="https://2014.igem.org/Team:UCL/Project/Biobricks">Lignin Peroxidase</a> | In order to widen our Azo-degrading and de-colourising toolbox, we also set out to investigate whether we could incorporate the <a href="https://2014.igem.org/Team:UCL/Project/Biobricks">Lignin Peroxidase</a> | ||
<a href="http://parts.igem.org/Part:BBa_K1336007">(BBa_K500000)</a> BioBrick submitted by Tianjin iGEM 2010 into our project and provide further characterisation. After getting this BioBrick synthesised | <a href="http://parts.igem.org/Part:BBa_K1336007">(BBa_K500000)</a> BioBrick submitted by Tianjin iGEM 2010 into our project and provide further characterisation. After getting this BioBrick synthesised | ||
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PLEASE INSERT: https://2014.igem.org/File:Ligper_fig1.png | PLEASE INSERT: https://2014.igem.org/File:Ligper_fig1.png | ||
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<b>Figure 6 - <a href="http://parts.igem.org/Part:BBa_K1336007">BBa_K1336007</a> BsDyP Azo-degradation module is capable of degrading Reactive Black 5 (RB5) dye-contaminated waste waters. </b> Graph showing that in comparison to the plasmid free control, E.coli transformed with the BBa_K1336007 BsDyP Azo-degradation module is able to decolourise RB5 dye-contaminated LB media after being induced by 1mM IPTG. Inoculations were grown at 37 degrees and 180rpm for 24 hours and then left stationary for a further 24 hours at room temperature. OD680nm measurements were taken of the supernatant at the end of the 48 hour experiment. Error bars indicate SEM, n=2. | <b>Figure 6 - <a href="http://parts.igem.org/Part:BBa_K1336007">BBa_K1336007</a> BsDyP Azo-degradation module is capable of degrading Reactive Black 5 (RB5) dye-contaminated waste waters. </b> Graph showing that in comparison to the plasmid free control, E.coli transformed with the BBa_K1336007 BsDyP Azo-degradation module is able to decolourise RB5 dye-contaminated LB media after being induced by 1mM IPTG. Inoculations were grown at 37 degrees and 180rpm for 24 hours and then left stationary for a further 24 hours at room temperature. OD680nm measurements were taken of the supernatant at the end of the 48 hour experiment. Error bars indicate SEM, n=2. | ||
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<b>Conclusions: DANIEL TO FILL OUT</b> | <b>Conclusions: DANIEL TO FILL OUT</b> | ||
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Revision as of 13:18, 17 October 2014
DEGRADATION TAB
In order to determine the compatibility of our BBa_K1336003 BsDyP Azo-degradation module, we set out to investigate the growth performance of E.coli DH5α containing our BioBrick in different media. We then set out to investigate the functionality of our Azo-degradation module in decolourising several model Azo-dye contaminated waste-waters.PLEASE INSERT: https://2014.igem.org/File:BsDyP_Figure_1.png
Figure 1 - BBa_K1336003 BsDyP Azo-degradation module preserves growth performance of E.coli DH5α in LB media. Graph showing that E.coli transformed with the BBa_K1336003 BsDyP Azo-degradation module shows comparable growth the plasmid-free control in LB media. OD measured at 680nm and Time is shown in hours after incubation. Error bars indicate SEM, n=2.
PLEASE INSERT: https://static.igem.org/mediawiki/2014/7/7f/Bsdyp_figure_2.png
Figure 2 - BBa_K1336003 BsDyP Azo-degradation module is compatible with Acid Orange 7 (AO7) dye-contaminated waste waters. Graph showing that E.coli transformed with the BBa_K1336003 BsDyP Azo-degradation module is able to grow in LB media contaminated with AO7 dye. Please note that OD measurements are considerably higher in dye-contaminated waters due to the absorbance of the azo-dye. OD measured at 680nm and Time is shown in hours after incubation. Error bars indicate SEM, n=2.
PLEASE INSERT: https://static.igem.org/mediawiki/2014/d/d6/Bsdyp_figure3.png
Figure 3 - BBa_K1336003 BsDyP Azo-degradation module is compatible with Reactive Black 5 (RB5) dye-contaminated waste waters. Graph showing that E.coli transformed with the BBa_K1336003 BsDyP Azo-degradation module is able to grow in LB media contaminated with RB5 dye. Please note that OD measurements are considerably higher in dye-contaminated waters due to the absorbance of the azo-dye. OD measured at 680nm and Time is shown in hours after incubation. Error bars indicate SEM, n=2.
In order to determine the capabilities of our BBa_K1336003 and BBa_K1336007 BsDyP Azo-degradation module, DANIEL TO FILL OUT!
PLEASE INSERT: https://static.igem.org/mediawiki/2014/d/db/Bsdyp_fig4.png
Figure 4 - BBa_K1336007 BsDyP Azo-degradation module is capable of degrading Acid Orange 7 (AO7) dye-contaminated waste waters. Graph showing that in comparison to the plasmid free control, E.coli transformed with the BBa_K1336007 BsDyP Azo-degradation module is able to decolourise AO7 dye contaminated LB media after being induced by 1mM IPTG. Inoculations were grown at 37 degrees and 180rpm for 24 hours and then left stationary for a further 24 hours at room temperature. OD680nm measurements were taken of the supernatant at the end of the 48 hour experiment. Error bars indicate SEM, n=2.
PLEASE INSERT: https://static.igem.org/mediawiki/2014/4/47/Bsdyp_fig5.png
Figure 5 - BBa_K1336007 BsDyP Azo-degradation module is capable of degrading Reactive Black 5 (RB5) dye-contaminated waste waters. Graph showing that in comparison to the plasmid free control, E.coli transformed with the BBa_K1336007 BsDyP Azo-degradation module is able to decolourise RB5 dye-contaminated LB media after being induced by 1mM IPTG. Inoculations were grown at 37 degrees and 180rpm for 24 hours and then left stationary for a further 24 hours at room temperature. OD680nm measurements were taken of the supernatant at the end of the 48 hour experiment. Error bars indicate SEM, n=2.
LEAVE SOME SPACE - next paragraph
In order to widen our Azo-degrading and de-colourising toolbox, we also set out to investigate whether we could incorporate the Lignin Peroxidase (BBa_K500000) BioBrick submitted by Tianjin iGEM 2010 into our project and provide further characterisation. After getting this BioBrick synthesised
PLEASE INSERT: https://2014.igem.org/File:Ligper_fig1.png
Figure 6 - BBa_K1336007 BsDyP Azo-degradation module is capable of degrading Reactive Black 5 (RB5) dye-contaminated waste waters. Graph showing that in comparison to the plasmid free control, E.coli transformed with the BBa_K1336007 BsDyP Azo-degradation module is able to decolourise RB5 dye-contaminated LB media after being induced by 1mM IPTG. Inoculations were grown at 37 degrees and 180rpm for 24 hours and then left stationary for a further 24 hours at room temperature. OD680nm measurements were taken of the supernatant at the end of the 48 hour experiment. Error bars indicate SEM, n=2.
Conclusions: DANIEL TO FILL OUT
