Team:CU-Boulder/Notebook/Safety
From 2014.igem.org
(Difference between revisions)
Line 7: | Line 7: | ||
<h3>Organism and Biological Part Safety </h3> | <h3>Organism and Biological Part Safety </h3> | ||
- | <ul> | + | <ul><ul> |
- | <br> | + | <br><br> |
<h4>Escherichia coli K12 variants</h4> | <h4>Escherichia coli K12 variants</h4> | ||
K12 strains of E. coli are commonly used, biosafety level 1 organisms. They pose little risk to lab members or the environment. They can be potentially harmful if ingested so basic lab safety such as gloves and hand washing were implemented to ensure lab member safety. | K12 strains of E. coli are commonly used, biosafety level 1 organisms. They pose little risk to lab members or the environment. They can be potentially harmful if ingested so basic lab safety such as gloves and hand washing were implemented to ensure lab member safety. | ||
- | <br> | + | <br><br> |
<h4>M13 phage</h4> | <h4>M13 phage</h4> | ||
M13 phage are biosafety level 1 viruses. Phage only infect bacteria so the risk to humans is negligible. The helper phagemid have a weakened packaging signal which reduces their replication efficiency, however, these phage could infect E. coli and other bacterial species containing the F’ episome if the phage escaped laboratory containment. M13 phage are not synthetic organisms; their environmental impact if they escaped laboratory containment is small due to their disrupted packaging signal so they would be quickly outcompeted by wild-type phage. Precautions such as hand washing and use of gloves were implemented to reduce risk to lab members. Proper disposal of samples was implemented to avoid environmental contamination. | M13 phage are biosafety level 1 viruses. Phage only infect bacteria so the risk to humans is negligible. The helper phagemid have a weakened packaging signal which reduces their replication efficiency, however, these phage could infect E. coli and other bacterial species containing the F’ episome if the phage escaped laboratory containment. M13 phage are not synthetic organisms; their environmental impact if they escaped laboratory containment is small due to their disrupted packaging signal so they would be quickly outcompeted by wild-type phage. Precautions such as hand washing and use of gloves were implemented to reduce risk to lab members. Proper disposal of samples was implemented to avoid environmental contamination. | ||
- | <br> | + | <br><br> |
<h4>Endogenous CRISPR-Cas(BBa_K1218011)</h4> | <h4>Endogenous CRISPR-Cas(BBa_K1218011)</h4> | ||
Part BBa_K1218011 was apart of the 2014 iGEM distribution. The encoded CRISPR-Cas9 system is derived from Streptococcus pyogenes, a biosafety level 1 organism, and is widely used in gene editing. Malicious use of the CRISPR-Cas9 system is theoretically possible as it is a system that targets specific genetic sequences and could be used to target certain alleles. However, this technology has been used for several years for the purpose of gene editing and no health risks have been reported. | Part BBa_K1218011 was apart of the 2014 iGEM distribution. The encoded CRISPR-Cas9 system is derived from Streptococcus pyogenes, a biosafety level 1 organism, and is widely used in gene editing. Malicious use of the CRISPR-Cas9 system is theoretically possible as it is a system that targets specific genetic sequences and could be used to target certain alleles. However, this technology has been used for several years for the purpose of gene editing and no health risks have been reported. | ||
- | </ul> | + | </ul></ul> |
<br> | <br> |
Revision as of 11:47, 17 October 2014