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Team:SZU-China
From 2014.igem.org
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<li id="menu-item-899" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-899"><a href="https://2014.igem.org/Team:SZU-China/Project/Kil">Kil Protein</a></li> | <li id="menu-item-899" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-899"><a href="https://2014.igem.org/Team:SZU-China/Project/Kil">Kil Protein</a></li> | ||
<li id="menu-item-900" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-900"><a href="https://2014.igem.org/Team:SZU-China/Project/ccdB">ccdB Suicide System</a></li> | <li id="menu-item-900" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-900"><a href="https://2014.igem.org/Team:SZU-China/Project/ccdB">ccdB Suicide System</a></li> | ||
| - | <li id="menu-item-901" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-901"><a href="https://2014.igem.org/Team:SZU-China/Project/ | + | <li id="menu-item-901" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-901"><a href="https://2014.igem.org/Team:SZU-China/Project/Collaboration">Collaboration</a></li> |
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<li id="menu-item-997" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-997"><a href="https://2014.igem.org/Team:SZU-China/Team/member">Member</a></li> | <li id="menu-item-997" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-997"><a href="https://2014.igem.org/Team:SZU-China/Team/member">Member</a></li> | ||
<li id="menu-item-996" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-996"><a href="https://2014.igem.org/Team:SZU-China/Team/Attribution">Attribution</a></li> | <li id="menu-item-996" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-996"><a href="https://2014.igem.org/Team:SZU-China/Team/Attribution">Attribution</a></li> | ||
| - | + | <li id="menu-item-940" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-940"><a href="https://2014.igem.org/Team:SZU-China/Team/Acknowledgment">Acknowledgment</a></li> | |
| + | <li id="menu-item-939" class="menu-item menu-item-type-post_type menu-item-object-post menu-item-939"><a href="https://igem.org/Team.cgi?year=2014">Official team profile</a></li> | ||
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<div class="category-cloud"><a>What we do</a></div> | <div class="category-cloud"><a>What we do</a></div> | ||
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<div class="col-md-5"> | <div class="col-md-5"> | ||
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| + | <h3> <b>Super Dream Factory of Cellulase </b></h3> | ||
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<figure class="thumbnail zan-thumb"><img width="450" height="240" src="https://static.igem.org/mediawiki/2014/2/28/SzuHome33.jpg" class="lazy attachment-medium wp-post-image" alt="banner_7" /> </figure> | <figure class="thumbnail zan-thumb"><img width="450" height="240" src="https://static.igem.org/mediawiki/2014/2/28/SzuHome33.jpg" class="lazy attachment-medium wp-post-image" alt="banner_7" /> </figure> | ||
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| + | <p style="font-size:large">We have made a super bacteria-Super Dream Factory of Cellulase.The factory has four parts. They are alkaline cellulase, Mdfa antiporter, kil protein and ccdB suicide system. <br>To enhance the effects of decontamination in various fields, we use an alkaline cellulase as detergent additive. The alkaline cellulase gene was introduced into the Escherichia coli and the target enzyme can be highly produced with the help of Kil, which is one of the core elements of our project. What’s more, MdfA, a proton pump across the cell membrane, was added to transport the proton into the cytoplasma, and release Na<sup>+</sup>/K<sup>+</sup> at the same time, resulting in cell survival in alkaline industry wastewaters as high as pH 9.5. Another highlight of our project is that we also added a ccdB suicide system to our e-machine, by this we could remove the biological machine whenever we need for biological safety. </p> | ||
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Revision as of 10:08, 17 October 2014
Super Dream Factory of Cellulase
We have made a super bacteria-Super Dream Factory of Cellulase.The factory has four parts. They are alkaline cellulase, Mdfa antiporter, kil protein and ccdB suicide system.
To enhance the effects of decontamination in various fields, we use an alkaline cellulase as detergent additive. The alkaline cellulase gene was introduced into the Escherichia coli and the target enzyme can be highly produced with the help of Kil, which is one of the core elements of our project. What’s more, MdfA, a proton pump across the cell membrane, was added to transport the proton into the cytoplasma, and release Na+/K+ at the same time, resulting in cell survival in alkaline industry wastewaters as high as pH 9.5. Another highlight of our project is that we also added a ccdB suicide system to our e-machine, by this we could remove the biological machine whenever we need for biological safety.
From SZU-China
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©2014 SZU-China
©2014 SZU-China
