Team:Caltech/week4
From 2014.igem.org
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<b>Export Systems</b> | <b>Export Systems</b> | ||
- | <ul><li>Discovered that the reverse primer used in colony PCR last night had no binding site on the plasmids we had assembled (pTG001 constructs)</li> | + | <ul><li>Discovered that the reverse primer used in colony PCR last night had no binding site on the plasmids we had assembled (pTG001 constructs), explaining the absence of any bands on the gels used for colony PCR</li> |
<li>Miniprepped liquid cultures grown up last night to extract plasmids | <li>Miniprepped liquid cultures grown up last night to extract plasmids | ||
<ul><li>Not all bacteria were able to be resuspended after pelleting, so resulting DNA yields for 6 of the 10 liquid cultures grown were very low (10-25 ng/uL)</li></ul> | <ul><li>Not all bacteria were able to be resuspended after pelleting, so resulting DNA yields for 6 of the 10 liquid cultures grown were very low (10-25 ng/uL)</li></ul> | ||
</li> | </li> | ||
+ | <li>Decided to scrap the last 2 day's experiments and ordered proper forward and reverse primers for colony PCR of pTG001 plasmids to be done tomorrow</li> | ||
</ul> | </ul> | ||
<b>Combinatorial Promoters</b> | <b>Combinatorial Promoters</b> | ||
- | <ul><li>Analyzed sequencing results and grew more liquid cultures of the colonies containing properly assembled plasmids</li> | + | <ul><li>Analyzed sequencing results and grew more overnight liquid cultures of the colonies containing properly assembled plasmids</li> |
</ul> | </ul> | ||
<br> | <br> |
Revision as of 18:15, 10 July 2014
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