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Team:CityU HK/project/result
From 2014.igem.org
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| - | <li><a href='https://2014.igem.org/Team:CityU_HK/project/result'><span>Result | + | <li><a href='https://2014.igem.org/Team:CityU_HK/project/result'><span>Result Summary</span></a></li> |
<li><a href='https://2014.igem.org/Team:CityU_HK/project/result_fad'><span>FadD & FadL Module</span></a></li> | <li><a href='https://2014.igem.org/Team:CityU_HK/project/result_fad'><span>FadD & FadL Module</span></a></li> | ||
<li><a href='https://2014.igem.org/Team:CityU_HK/project/result_tesA'><span>'TesA Module</span></a></li> | <li><a href='https://2014.igem.org/Team:CityU_HK/project/result_tesA'><span>'TesA Module</span></a></li> | ||
| - | <li class='last'><a href=' | + | <li class='last'><a href='https://2014.igem.org/Team:CityU_HK/project/result_desaturase'><span>Desaturase Module</span></a></li> |
</ul> | </ul> | ||
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| + | <div><img src="https://static.igem.org/mediawiki/2014/b/bc/CityU_HK_result-banner.png" width="100%"></div> | ||
<h1 id="title"> Result Summary </h1> | <h1 id="title"> Result Summary </h1> | ||
<h2 class="sub">Experimental Achievements from 15 June – 3 October 2014 :</h2> | <h2 class="sub">Experimental Achievements from 15 June – 3 October 2014 :</h2> | ||
| - | <p class="content">Successfully constructed a Biobrick for a tetracycline regulated fadL-fadD expression system (<a href="http://parts.igem.org/Part: | + | <div class="container"><div class="row"> |
| - | Successfully constructed and characterized a T7-regulated fadL-fadD system in the pSNAP-tag® (T7)-2 expression vector.<br><br> | + | <div class="col-md-12"> |
| - | Successfully constructed a Biobrick containing a “leaderless” tesA coding sequence (‘tesA) (<a href="http://parts.igem.org/Part:BBa_K1472601" target="_blank">BBa_K1472601</a>).<br><br> | + | <p class="content">Successfully constructed a Biobrick for a tetracycline regulated <i>fadL-fadD</i> expression system (<a href="http://parts.igem.org/Part:BBa_K1472606" target="_blank">BBa_K1472606</a>).<br><br> |
| - | Successfully constructed a translational unit of the ‘tesA gene in pAB1A2. <br><br> | + | Successfully constructed and characterized a T7-regulated <i>fadL-fadD</i> system in the pSNAP-tag® (T7)-2 expression vector.<br><br> |
| - | Successfully constructed an arabinose-regulated ‘tesA expression system in pSB1C3.<br><br> | + | Successfully constructed a Biobrick containing a “leaderless” <i>tesA</i> coding sequence (<i>‘tesA</i>) (<a href="http://parts.igem.org/Part:BBa_K1472601" target="_blank">BBa_K1472601</a>).<br><br> |
| + | Successfully constructed a translational unit of the <i>‘tesA</i> gene in pAB1A2. <br><br> | ||
| + | Successfully constructed an arabinose-regulated <i>‘tesA</i> expression system in pSB1C3.<br><br> | ||
Successfully constructed a Biobrick containing a Δ15 desaturase gene that is codon-optimized for protein expression in E.coli (<a href="http://parts.igem.org/Part:BBa_K1472610" target="_blank">BBa_K1472610</a>).<br><br> | Successfully constructed a Biobrick containing a Δ15 desaturase gene that is codon-optimized for protein expression in E.coli (<a href="http://parts.igem.org/Part:BBa_K1472610" target="_blank">BBa_K1472610</a>).<br><br> | ||
Successfully constructed a translational unit of a Δ9 desaturase gene in pSB1A2 that is codon-optimized for E. coli.<br><br> | Successfully constructed a translational unit of a Δ9 desaturase gene in pSB1A2 that is codon-optimized for E. coli.<br><br> | ||
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Successfully constructed a translational unit of a Δ9-Δ12-Δ15 desaturase gene cluster in pSB1A2. | Successfully constructed a translational unit of a Δ9-Δ12-Δ15 desaturase gene cluster in pSB1A2. | ||
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Latest revision as of 02:31, 17 October 2014
Result Summary
Experimental Achievements from 15 June – 3 October 2014 :
Successfully constructed a Biobrick for a tetracycline regulated fadL-fadD expression system (BBa_K1472606).
Successfully constructed and characterized a T7-regulated fadL-fadD system in the pSNAP-tag® (T7)-2 expression vector.
Successfully constructed a Biobrick containing a “leaderless” tesA coding sequence (‘tesA) (BBa_K1472601).
Successfully constructed a translational unit of the ‘tesA gene in pAB1A2.
Successfully constructed an arabinose-regulated ‘tesA expression system in pSB1C3.
Successfully constructed a Biobrick containing a Δ15 desaturase gene that is codon-optimized for protein expression in E.coli (BBa_K1472610).
Successfully constructed a translational unit of a Δ9 desaturase gene in pSB1A2 that is codon-optimized for E. coli.
Successfully constructed a translational unit of a Δ12 desaturase gene in pSB1A2 that is codon-optimized for E. coli.
Successfully constructed a translational unit of a Δ15 desaturase gene in pSB1A2 that is codon-optimized for E. coli.
Successfully constructed a translational unit of a Δ9-Δ12 desaturase gene cluster in pSB1A2.
Successfully constructed a translational unit of a Δ9-Δ12-Δ15 desaturase gene cluster in pSB1A2.
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