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Team:Cambridge-JIC/Guide/Materials/Checklist
From 2014.igem.org
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==DNA== | ==DNA== | ||
| - | - A [[Team:Cambridge-JIC/Guide/Constructs/AgrobacteriumVectors| helper plasmid]] for [[Team:Cambridge-JIC/Guide/Transformation|Transformation] | + | - A [[Team:Cambridge-JIC/Guide/Constructs/AgrobacteriumVectors| helper plasmid]] for [[Team:Cambridge-JIC/Guide/Transformation|Transformation]] |
- Your [[Team:Cambridge-JIC/Guide/Constructs|T-DNA plasmid]] for transformation | - Your [[Team:Cambridge-JIC/Guide/Constructs|T-DNA plasmid]] for transformation | ||
| + | |||
==[[Team:Cambridge-JIC/Guide/Materials/Strains |Bacteria]]== | ==[[Team:Cambridge-JIC/Guide/Materials/Strains |Bacteria]]== | ||
- Competent E-Coli (chemically/electro) | - Competent E-Coli (chemically/electro) | ||
Revision as of 00:34, 17 October 2014
Contents |
Checklist
This checklist contains everything you will need for setting up transformation.
DNA
- A helper plasmid for Transformation
- Your T-DNA plasmid for transformation
Bacteria
- Competent E-Coli (chemically/electro)
- Electrocompetent agrobacteria
Marchantia
OR
- Male and female Marchantia polymorpha [from the garden]
Miscellaneous
- LB/SOC for bacterial transformation and growth
- 100mM Acetosyringone
Equipment
- Ventilated cupboard/room/box under full illumination [larger space = more transformations]
