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Team:Cambridge-JIC/Guide/Materials/Checklist
From 2014.igem.org
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==DNA== | ==DNA== | ||
- A [[Team:Cambridge-JIC/Guide/Constructs/AgrobacteriumVectors| helper plasmid]] for [[Team:Cambridge-JIC/Guide/Transformation|Transformation] | - A [[Team:Cambridge-JIC/Guide/Constructs/AgrobacteriumVectors| helper plasmid]] for [[Team:Cambridge-JIC/Guide/Transformation|Transformation] | ||
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- Your [[Team:Cambridge-JIC/Guide/Constructs|T-DNA plasmid]] for transformation | - Your [[Team:Cambridge-JIC/Guide/Constructs|T-DNA plasmid]] for transformation | ||
==[[Team:Cambridge-JIC/Guide/Materials/Strains |Bacteria]]== | ==[[Team:Cambridge-JIC/Guide/Materials/Strains |Bacteria]]== | ||
- Competent E-Coli (chemically/electro) | - Competent E-Coli (chemically/electro) | ||
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- [[Team:Cambridge-JIC/Guide/Transformation/PreparingElectrocompetentAgro | Electrocompetent agrobacteria]] | - [[Team:Cambridge-JIC/Guide/Transformation/PreparingElectrocompetentAgro | Electrocompetent agrobacteria]] | ||
==Marchantia== | ==Marchantia== | ||
- [[Team:Cambridge-JIC/Guide/Care/PreparingSpores| Sporangia]] | - [[Team:Cambridge-JIC/Guide/Care/PreparingSpores| Sporangia]] | ||
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OR | OR | ||
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- Male and female Marchantia polymorpha [from the garden] | - Male and female Marchantia polymorpha [from the garden] | ||
==Miscellaneous== | ==Miscellaneous== | ||
- [[Team:Cambridge-JIC/Guide/Materials/Media|Gamborg's B5 salt mix]] | - [[Team:Cambridge-JIC/Guide/Materials/Media|Gamborg's B5 salt mix]] | ||
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- LB/SOC for bacterial transformation and growth | - LB/SOC for bacterial transformation and growth | ||
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- 100mM Acetosyringone | - 100mM Acetosyringone | ||
==Equipment== | ==Equipment== | ||
- Ventilated cupboard/room/box under full illumination [larger space = more transformations] | - Ventilated cupboard/room/box under full illumination [larger space = more transformations] | ||
Revision as of 00:33, 17 October 2014
Contents |
Checklist
This checklist contains everything you will need for setting up transformation.
DNA
- A helper plasmid for [[Team:Cambridge-JIC/Guide/Transformation|Transformation]
- Your T-DNA plasmid for transformation
Bacteria
- Competent E-Coli (chemically/electro)
- Electrocompetent agrobacteria
Marchantia
OR
- Male and female Marchantia polymorpha [from the garden]
Miscellaneous
- LB/SOC for bacterial transformation and growth
- 100mM Acetosyringone
Equipment
- Ventilated cupboard/room/box under full illumination [larger space = more transformations]
