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Team:Cambridge-JIC/Guide/Materials/Checklist
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(Created page with "*** Checklist This checklist contains everything you will need for setting up transformation **** DNA - A helper plasmid for agrobacterium mediated transformation - Your [[T-...") |
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| - | + | =Checklist= | |
| - | This checklist contains everything you will need for setting up transformation | + | This checklist contains everything you will need for setting up transformation. |
| - | + | ||
| - | - A [[helper plasmid]] for | + | ==DNA== |
| - | - Your [[T-DNA]] | + | - A [[Team:Cambridge-JIC/Guide/Constructs/AgrobacteriumVectors| helper plasmid]] for [[Team:Cambridge-JIC/Guide/Transformation|Transformation] |
| - | + | - Your [[Team:Cambridge-JIC/Guide/Constructs|T-DNA plasmid]] for transformation | |
| + | ==[[Team:Cambridge-JIC/Guide/Materials/Strains |Bacteria]]== | ||
- Competent E-Coli (chemically/electro) | - Competent E-Coli (chemically/electro) | ||
| - | - [[Electrocompetent agrobacteria]] | + | - [[Team:Cambridge-JIC/Guide/Transformation/PreparingElectrocompetentAgro | Electrocompetent agrobacteria]] |
| - | + | ==Marchantia== | |
| - | - [[ | + | - [[Team:Cambridge-JIC/Guide/Care/PreparingSpores| Sporangia]] |
OR | OR | ||
- Male and female Marchantia polymorpha [from the garden] | - Male and female Marchantia polymorpha [from the garden] | ||
| - | + | ==Miscellaneous== | |
| - | - Gamborg's B5 salt mix | + | - [[Team:Cambridge-JIC/Guide/Materials/Media|Gamborg's B5 salt mix]] |
- LB/SOC for bacterial transformation and growth | - LB/SOC for bacterial transformation and growth | ||
- 100mM Acetosyringone | - 100mM Acetosyringone | ||
| - | + | ||
| + | ==Equipment== | ||
- Ventilated cupboard/room/box under full illumination [larger space = more transformations] | - Ventilated cupboard/room/box under full illumination [larger space = more transformations] | ||
Revision as of 00:32, 17 October 2014
Contents |
Checklist
This checklist contains everything you will need for setting up transformation.
DNA
- A helper plasmid for [[Team:Cambridge-JIC/Guide/Transformation|Transformation] - Your T-DNA plasmid for transformation
Bacteria
- Competent E-Coli (chemically/electro) - Electrocompetent agrobacteria
Marchantia
- Sporangia OR - Male and female Marchantia polymorpha [from the garden]
Miscellaneous
- Gamborg's B5 salt mix - LB/SOC for bacterial transformation and growth - 100mM Acetosyringone
Equipment
- Ventilated cupboard/room/box under full illumination [larger space = more transformations]
