Team:SF Bay Area DIYbio/Notebook

From 2014.igem.org

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Notebook
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<p><b>Notebook</b></p>
<p><b>General Protocols</b></p>
<p><b>General Protocols</b></p>
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     </ul>
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<p>June 2014</p><ul>
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<p><b>June 2014</b></p><ul>
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     <li><a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/NoteBookJune1">CR_062214_humanKappaCasein_001</a> <br></ul></p>
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     <li><a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/NoteBookJune1">CR_062214_humanKappaCasein_001</a> <br></ul></p><br>
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<p>August 2014</p><ul>
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<p><b>August 2014</b></p><ul>
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     <li>CLONE_082514_humKappaCasein(Kex+)<br>
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     <li><a href="https://2014.igem.org/Team:SF_Bay_Area_DIYbio/AugustCloning">CLONE_082514_humKappaCasein(Kex+)</a><br>
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     <li>Growth Curve Experiment <br></ul></p>
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     <li>Growth Curve Experiment <br></ul></p><br>
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<p>September 2014</p><ul>
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<p><b>September 2014</b></p><ul>
     <li>Cloning 7 casein gBlocks, 12Sep2014 and on<br>
     <li>Cloning 7 casein gBlocks, 12Sep2014 and on<br>
     <li>Troubleshooting failed 12Sep2014 transformation of P.FAKS.humBeta.S<br>
     <li>Troubleshooting failed 12Sep2014 transformation of P.FAKS.humBeta.S<br>
     <li>Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014<br>
     <li>Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014<br>
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     <li>Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014<br></ul></p>
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     <li>Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014<br></ul></p><br>
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<p>October 2014</p><ul>
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<p><b>October 2014</b></p><ul>
     <li>Repeat Midipreps to select new clones for sequencing for failed sequences from Oct. 2<br>
     <li>Repeat Midipreps to select new clones for sequencing for failed sequences from Oct. 2<br>
     <li>Cloning human alpha casein S1, 04Oct2014 and on<br>
     <li>Cloning human alpha casein S1, 04Oct2014 and on<br>

Revision as of 20:05, 16 October 2014



WELCOME TO iGEM 2014!

Your team has been approved and you are ready to start the iGEM season!
On this page you can document your project, introduce your team members, document your progress
and share your iGEM experience with the rest of the world!


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Home Team Official Team Profile Project Parts Modeling Notebook Safety Attributions

Notebook

General Protocols


  • Electra Cloning Protocol
  • DNA Handling Protocol
  • Media:Zymo E. coli competent cells transformation protocol.pdf
  • Media:D4025i.pdf (Zyppy plasmid midiprep protocol)
  • Media:Clontech UTF-8'en-us'Quick Easy Yeast Transformation Mix Protocol-At-A-Glance 040214.pdf

General Documentation Information

June 2014


August 2014


September 2014

  • Cloning 7 casein gBlocks, 12Sep2014 and on
  • Troubleshooting failed 12Sep2014 transformation of P.FAKS.humBeta.S
  • Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014
  • Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014


October 2014

  • Repeat Midipreps to select new clones for sequencing for failed sequences from Oct. 2
  • Cloning human alpha casein S1, 04Oct2014 and on
  • Submitting 4 repeat and 1 new casein in pD1214 plasmid for sequencing 10Oct2014

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