Team:Aachen/Achievements
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We developed and optimized a novel [https://2014.igem.org/Team:Aachen/Project/2D_Biosensor method] of reading out biosensors on a two dimensional level. The sensor cells are therefore immobilized in an agar chip. It was experimentally [https://2014.igem.org/Team:Aachen/Project/2D_Biosensor#biosensorachievements validated] that this method works for different kinds of reporter cells, induction substances and readout methods including our newly designed system [https://2014.igem.org/Team:Aachen/Project/Measurement_Device ''WatsOn'']. | We developed and optimized a novel [https://2014.igem.org/Team:Aachen/Project/2D_Biosensor method] of reading out biosensors on a two dimensional level. The sensor cells are therefore immobilized in an agar chip. It was experimentally [https://2014.igem.org/Team:Aachen/Project/2D_Biosensor#biosensorachievements validated] that this method works for different kinds of reporter cells, induction substances and readout methods including our newly designed system [https://2014.igem.org/Team:Aachen/Project/Measurement_Device ''WatsOn'']. | ||
- | To improve the response of our reporter cells to induction we designed and engineered a novel [https://2014.igem.org/Team:Aachen/Project/FRET_Reporter reporter system] using a TEV protease to activate a previously synthesized reporter protein by cleaning off a quencher. A faster response in comparison to regular expression of the reporter protein was shown by modeling as well as in our experiments. All parts needed to build this kind of reporter system were submitted to the partsregistry. | + | To improve the response of our reporter cells to induction we designed and engineered a novel [https://2014.igem.org/Team:Aachen/Project/FRET_Reporter reporter system] using a TEV protease to activate a previously synthesized reporter protein by cleaning off a quencher. A faster response in comparison to regular expression of the reporter protein was shown by [https://2014.igem.org/Team:Aachen/Project/Model modeling] as well as in our experiments. All parts needed to build this kind of reporter system were submitted to the partsregistry. |
{{Team:Aachen/BlockSeparator}} | {{Team:Aachen/BlockSeparator}} |
Revision as of 18:46, 16 October 2014
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