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Team:TU Darmstadt/Notebook/Materials/Media
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Revision as of 16:28, 16 October 2014
Media
1.7-DYT (recipe for 500 mL)
- 2.5 g NaCl
- 8.0 g tryptone
- 5.0 g yeast extract
- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)
LB (recipe for 1000 mL)
- 10 g tryptone
- 5 g yeast extract
- 10 g NaCl
- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)
For preparing plates add 15 g agar.
SOB (recipe for 1000 mL)
- 0.186 g KCl
- 2.4 g MgSO4
- 0.584 g NaCl
- 20 g tryptone
- 5 g yeast extract
- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)
SOC (recipe for 1000 mL)
- 980 mL SOB
- 20 mL 1M Glucose
Autoclave after mixing.
