Team:Bielefeld-CeBiTec/Notebook/Journal/Isobutanol/Jun

From 2014.igem.org

(Difference between revisions)
Line 56: Line 56:
                <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Transformationviaelectroporation" target="_blank">Transformation</a> with electrocompotetent cells</li>
                <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Transformationviaelectroporation" target="_blank">Transformation</a> with electrocompotetent cells</li>
                         <ul>
                         <ul>
-
                           <li>K539627: pSB1C3-<i>alsS</i> (parts distribution from 2013, plate 1, 24H)</li>
+
                           <li><a href="http://parts.igem.org/Part:BBa_K539627" target="_blank">K539627</a>: pSB1C3-<i>alsS</i> (parts distribution from 2013, plate 1, 24H)</li>
                         </ul>
                         </ul>
                         <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of <i>alsS</i></li>
                         <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of <i>alsS</i></li>
Line 69: Line 69:
                <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Transformationviaelectroporation" target="_blank">Transformation</a> with electrocompotetent cells</li>
                <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Transformationviaelectroporation" target="_blank">Transformation</a> with electrocompotetent cells</li>
                         <ul>
                         <ul>
-
                           <li>K539621: pSB1C3-<i>ilvC</i> (parts distribution from 2013, plate 1, 24F)</li>
+
                           <li><a href="http://parts.igem.org/Part:BBa_K539621" target="_blank">K539621</a>: pSB1C3-<i>ilvC</i> (parts distribution from 2013, plate 1, 24F)</li>
                         </ul>
                         </ul>
                         <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of <i>ilC</i></li>
                         <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of <i>ilC</i></li>
Line 82: Line 82:
                <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Transformationviaelectroporation" target="_blank">Transformation</a> with electrocompotetent cells</li>
                <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Transformationviaelectroporation" target="_blank">Transformation</a> with electrocompotetent cells</li>
                         <ul>
                         <ul>
-
                           <li>K539626: pSB1C3-<i>ilvD</i> (parts distribution from 2013, plate 1, 22J)</li>
+
                           <li><a href="http://parts.igem.org/Part:BBa_K539626" target="_blank">K539626</a>: pSB1C3-<i>ilvD</i> (parts distribution from 2013, plate 1, 22J)</li>
                         </ul>
                         </ul>
                         <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of <i>ilvD</i></li>
                         <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of <i>ilvD</i></li>
Line 95: Line 95:
                <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Transformationviaelectroporation" target="_blank">Transformation</a> with electrocompotetent cells</li>
                <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Transformationviaelectroporation" target="_blank">Transformation</a> with electrocompotetent cells</li>
                         <ul>
                         <ul>
-
                           <li>K538742: pSB1C3-<i>kivD</i> (parts distribution from 2013, plate 1, 24L)</li>
+
                           <li><a href="http://parts.igem.org/Part:BBa_K539742" target="_blank">K539742</a>: pSB1C3-<i>kivD</i> (parts distribution from 2013, plate 1, 24L)</li>
                         </ul>
                         </ul>
                         <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of <i>kivD</i></li>
                         <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of <i>kivD</i></li>

Revision as of 15:45, 16 October 2014


June

  • alsS
    • This week we aim to transform the construct from the parts distribution.
      • Transformation with electrocompotetent cells
        • K539627: pSB1C3-alsS (parts distribution from 2013, plate 1, 24H)
      • Plasmid isolation of alsS
      • Sequencing confirmed the identity of pSB1C3-alsS, but not the whole sequence of this part was covered. (VF, VR)
      • Glycerin stock created: E. coli KRX pSB1C3-alsS
  • ilvC
    • This week we aim to transform the construct from the parts distribution.
      • Transformation with electrocompotetent cells
        • K539621: pSB1C3-ilvC (parts distribution from 2013, plate 1, 24F)
      • Plasmid isolation of ilC
      • Sequencing confirmed the identity of pSB1C3-ilvC, but not the whole sequence of this part was covered. (VF, VR)
      • Glycerin stock created: E. coli KRX pSB1C3-ilvC
  • ilvD
    • This week we aim to transform the construct from the parts distribution.
      • Transformation with electrocompotetent cells
        • K539626: pSB1C3-ilvD (parts distribution from 2013, plate 1, 22J)
      • Plasmid isolation of ilvD
      • Sequencing confirmed the identity of pSB1C3-ilvD, but not the whole sequence of this part was covered. (VF, VR)
      • Glycerin stock created: E. coli KRX pSB1C3-ilvD
  • kivD
    • This week we aim to transform the construct from the parts distribution.
      • Transformation with electrocompotetent cells
        • K539742: pSB1C3-kivD (parts distribution from 2013, plate 1, 24L)
      • Plasmid isolation of kivD
      • Sequencing confirmed the identity of pSB1C3-kivD, but not the whole sequence of this part was covered. (VF, VR)
      • Glycerin stock created: E. coli KRX pSB1C3-kivD