"
Team:UFAM Brazil/Protocol2
From 2014.igem.org
(Difference between revisions)
Manickchand (Talk | contribs) |
Manickchand (Talk | contribs) |
||
| (One intermediate revision not shown) | |||
| Line 9: | Line 9: | ||
<tr><td><h1>Cryopreservation of bacteria</h1></td></tr> | <tr><td><h1>Cryopreservation of bacteria</h1></td></tr> | ||
| - | <tr><td colspan="3" width=" | + | <tr><td colspan="3" width="960"> |
<p><b>Reagents:</b> </p> | <p><b>Reagents:</b> </p> | ||
| Line 26: | Line 26: | ||
<tr> | <tr> | ||
| - | <td class=" | + | <td class="backProtocols" ><a href="https://2014.igem.org/Team:UFAM_Brazil/Methods">Protocols</a></td> |
| - | + | ||
| - | + | ||
| + | </tr> | ||
</table> | </table> | ||
</body> | </body> | ||
</html> | </html> | ||
Latest revision as of 15:22, 16 October 2014
Cryopreservation of bacteria | ||
|
Reagents: - Glycerol 50% Steps: 1. Grow bacteria in liquid LB medium until the optical density in a spectrophotometer (600 nm) of 0.5 - 0.8 abs. 2. Add to the required amount of bacteria 1 volume of 50% glycerol in 1.5ml microtubes. 3. Cryopreservation temperature at -80 ° C. | ||
| Protocols | ||
