Team:Tokyo-NoKoGen

From 2014.igem.org

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<p>Cockroaches and many other insects store their energy in the form of trehalose and use the enzyme trehalase to convert it to glucose when needed. Our engineered <i>E. coli</i> will overexpress the enzymes OtsA and OtsB to produce trehalose, and glucose-3-dehydrogenase to convert trehalose to 3,3’-diketotrehalose, an inhibitor of trehalase that is not toxic for mammals. Our engineered Exterminator coli will help eradicate roaches from homes without harming humans or pets.</p>-->
<p>Cockroaches and many other insects store their energy in the form of trehalose and use the enzyme trehalase to convert it to glucose when needed. Our engineered <i>E. coli</i> will overexpress the enzymes OtsA and OtsB to produce trehalose, and glucose-3-dehydrogenase to convert trehalose to 3,3’-diketotrehalose, an inhibitor of trehalase that is not toxic for mammals. Our engineered Exterminator coli will help eradicate roaches from homes without harming humans or pets.</p>-->
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<p>Pesticide to harmful insects, such as roaches, puts human and environment at risk, so more safety pesticide is needed. Our project for iGEM 2014 is “Exterminator coli”. We engineered <i>E.coli</i> that produces an inhibitor of metabolic pathway for insects and exterminates insects such as roaches. In our project, we can produce pesticide that has no effect for mammals and environment by just one kind of <i>E.coli</i>. </p><br>
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<p>Pesticides that are harmful to insects, such as cockroaches, put humans and the environment at risk, so safety pesticides are needed. Our project for iGEM 2014 is “Exterminator coli”. We engineered <i>Escherichia coli</i> (<i>E.coli</i>) that produces an inhibitor of a metabolic pathway for insects and exterminates insects such as roaches. In our project, we will produce a pesticide that has no effect on mammals and the environment using <i>E.coli</i>. </p><br>
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<p>Roaches and many other insects store their energy in the form of trehalose and use the enzyme, “trehalase” which converts trehalose to glucose when needed. While, mammals store their energy in the form of glycogen, so trehalase is not used for conversion it to glucose. Therefore trehalase inhibitor is used as pesticide which isn’t harmful to mammals but roaches. </p><br>
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<p>Roaches and many other insects store their energy in the form of trehalose and use the enzyme trehalase to convert trehalose to glucose when needed. However, mammals store their energy in the form of glycogen, which does not require trehalase for conversion to glucose. Therefore trehalase inhibitor can be used as a pesticide which isn’t harmful to mammals but roaches. </p><br>
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<p>We engineered E.coli that produces trehalase inhibitor, 3, 3´-diketotorehalose (3, 3´-dkT), as harmless pesticide to environment and mammals. Our engineered <i>E. coli </i>will overexpress <i>otsA</i> and <i>otsB</i> which produce enzymes convert glucose-6-phosphate and UDP-glucose to trehalose, and glucose-3-dehydrogenase (G3DH) which converts trehalose to 3, 3’-dkT. Therefore our <i>E.coli</i> can continue to produce 3, 3’-dkT by using glucose in insects. Just what we do is that make roaches eat <i>E.coli</i> we engineered.</p>
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<p>We engineered <i>E.coli</i> that produces the trehalase inhibitor, 3, 3'-diketotrehalose (3, 3'-dkT), a harmless pesticide to the environment and mammals. Our engineered <i>E.coli</i> will overexpress <i>OtsA</i> and <i>OtsB</i> which convert glucose-6-phosphate and UDP-glucose to trehalose, and glucose-3-dehydrogenase (G3DH) which converts trehalose to 3, 3'-dkT. Therefore, we predict that our <i>E.coli</i> can continue to produce 3, 3'-dkT by using glucose in insects. All we need to do is make roaches eat the <i>E.coli</i> we engineered !</p>
<img src="https://static.igem.org/mediawiki/2014/4/4a/NoKo14_Ecoli.jpg"><br>
<img src="https://static.igem.org/mediawiki/2014/4/4a/NoKo14_Ecoli.jpg"><br>

Revision as of 13:47, 16 October 2014

Overview

Pesticides that are harmful to insects, such as cockroaches, put humans and the environment at risk, so safety pesticides are needed. Our project for iGEM 2014 is “Exterminator coli”. We engineered Escherichia coli (E.coli) that produces an inhibitor of a metabolic pathway for insects and exterminates insects such as roaches. In our project, we will produce a pesticide that has no effect on mammals and the environment using E.coli.


Roaches and many other insects store their energy in the form of trehalose and use the enzyme trehalase to convert trehalose to glucose when needed. However, mammals store their energy in the form of glycogen, which does not require trehalase for conversion to glucose. Therefore trehalase inhibitor can be used as a pesticide which isn’t harmful to mammals but roaches.


We engineered E.coli that produces the trehalase inhibitor, 3, 3'-diketotrehalose (3, 3'-dkT), a harmless pesticide to the environment and mammals. Our engineered E.coli will overexpress OtsA and OtsB which convert glucose-6-phosphate and UDP-glucose to trehalose, and glucose-3-dehydrogenase (G3DH) which converts trehalose to 3, 3'-dkT. Therefore, we predict that our E.coli can continue to produce 3, 3'-dkT by using glucose in insects. All we need to do is make roaches eat the E.coli we engineered !



Our E.coli products 3'3-diketotrehlose (red triangle) from glucose(yellow circle).
3'3-diketotrehalose inhibit the reaction of trehalase, which is one of carbon source in insects.

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