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Team:UFAM Brazil/Protocol2
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Revision as of 13:25, 16 October 2014
Cryopreservation of bacteria | ||
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Reagents: - Glycerol 50% Steps: 1. Grow bacteria in liquid LB medium until the optical density in a spectrophotometer (600 nm) of 0.5 - 0.8 abs. 2. Add to the required amount of bacteria 1 volume of 50% glycerol in 1.5ml microtubes. 3. Cryopreservation temperature at -80 ° C. | ||
| Methods | ||
