• Result Summary
• FadD & FadL Module
• 'TesA Module
• Desaturase Module

Result Summary

Experimental Achievements from 15 June – 3 October 2014 :

Successfully constructed a Biobrick for a tetracycline regulated fadL-fadD expression system (BBa_K1472606).

Successfully constructed and characterized a T7-regulated fadL-fadD system in the pSNAP-tag® (T7)-2 expression vector.

Successfully constructed a Biobrick containing a “leaderless” tesA coding sequence (‘tesA) (BBa_K1472601).

Successfully constructed a translational unit of the ‘tesA gene in pAB1A2.

Successfully constructed an arabinose-regulated ‘tesA expression system in pSB1C3.

Successfully constructed a Biobrick containing a Δ15 desaturase gene that is codon-optimized for protein expression in E.coli (BBa_K1472610).

Successfully constructed a translational unit of a Δ9 desaturase gene in pSB1A2 that is codon-optimized for E. coli.

Successfully constructed a translational unit of a Δ12 desaturase gene in pSB1A2 that is codon-optimized for E. coli.

Successfully constructed a translational unit of a Δ15 desaturase gene in pSB1A2 that is codon-optimized for E. coli.

Successfully constructed a translational unit of a Δ9-Δ12 desaturase gene cluster in pSB1A2.

Successfully constructed a translational unit of a Δ9-Δ12-Δ15 desaturase gene cluster in pSB1A2.