Team:Macquarie Australia/WetLab/Protocols/Electrophoresis

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<li>Photograph gels under UV light</li>
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<p><b>Figure 1.</b> Bio-Rad agarose gel.</p>
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Revision as of 08:19, 16 October 2014

Agarose Gel Electrophoresis


Preparing the Gel

  • Mix 1g of agarose powder with 100ml of 1x TAE buffer and heat for 1minute or until all agarose is dissolved.
  • Wait until it has cooled (not set), and add 1ul of GelRed into the mixture.
  • Pour the solution into a cast with an appropriate comb.
  • Leave to set.

Running the Gel

  • Mix 1ul of 1kbp DNA ladder with 6ul of loading dye (bromophenol blue) and 4ul of 1x TAE buffer (total 6ul) and load onto first well
  • Mix 5ul of PCR products with 1ul of loading dye and load onto wells.
  • Run gel at 90V for 45minutes approximately
  • Photograph gels under UV light

Figure 1. Bio-Rad agarose gel.