Team:Cornell/project/wetlab/futurework
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- | + | In the future, we hope to continue working with the <i>merT</i>, <i>merP</i>, <i>CBP4</i>, and <i>nixA</i> heavy metal transport genes by incorporating them upstream of the metallothionein gene <i>GST-YMT</i>. Once each heavy metal transport gene is combined with the metallothionein gene, we can transform the high copy bacterial plasmid into <i>E. coli</i>. We will then be able to conduct a series of growth assays between our engineered bacteria and <i>E. coli</i> in the presence of heavy metal contaminated water. | |
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- | + | We also hope to continue working on synthesizing a reporter system. In order to detect the saturation of metallothionein sequestering cultures, we plan on using <i>amilCP</i> behind the nickel/cobalt activated promoter <i>Prcn</i> and the mercury activated promoter <i>PmerT</i>. It would be useful to place <i>amilCP</i> behind a lead activated promoter. This system should be incorporated into the BioBrick backbone and transformed into <i>E. coli</i> reporter cultures. These would theoretically be placed into a second hollow fiber reactor that would be connected downstream to the transporter-metallothionein hollow fiber reactor. Effluent water carrying unsequestered metal ions would induce the reporter culture to express <i>amilCP</i>, producing a gradient of blue. We can then test water samples with different heavy metal concentrations to correlate effluent levels against the cultures’ color gradient. | |
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Revision as of 07:32, 16 October 2014
Wet Lab
![](https://static.igem.org/mediawiki/2014/9/92/Copy_of_Shells_at_Ithaca_Gun_Factory.jpg)
Future Work
In the future, we hope to continue working with the merT, merP, CBP4, and nixA heavy metal transport genes by incorporating them upstream of the metallothionein gene GST-YMT. Once each heavy metal transport gene is combined with the metallothionein gene, we can transform the high copy bacterial plasmid into E. coli. We will then be able to conduct a series of growth assays between our engineered bacteria and E. coli in the presence of heavy metal contaminated water.We also hope to continue working on synthesizing a reporter system. In order to detect the saturation of metallothionein sequestering cultures, we plan on using amilCP behind the nickel/cobalt activated promoter Prcn and the mercury activated promoter PmerT. It would be useful to place amilCP behind a lead activated promoter. This system should be incorporated into the BioBrick backbone and transformed into E. coli reporter cultures. These would theoretically be placed into a second hollow fiber reactor that would be connected downstream to the transporter-metallothionein hollow fiber reactor. Effluent water carrying unsequestered metal ions would induce the reporter culture to express amilCP, producing a gradient of blue. We can then test water samples with different heavy metal concentrations to correlate effluent levels against the cultures’ color gradient.