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Team:Macquarie Australia/WetLab/Protocols/Electrophoresis
From 2014.igem.org
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<li>Mix 1ul of 1kbp DNA ladder with 6ul of loading dye (bromophenol blue) and 4ul of 1x TAE buffer (total 6ul) and load onto first well </li> | <li>Mix 1ul of 1kbp DNA ladder with 6ul of loading dye (bromophenol blue) and 4ul of 1x TAE buffer (total 6ul) and load onto first well </li> | ||
Revision as of 04:44, 16 October 2014
Agarose Gel Electrophoresis
Preparing the Gel
-
Mix 1g of agarose powder with 100ml of 1x TAE buffer and heat for 1minute or until all agarose is dissolved.
-
Wait until it has cooled (not set), and add 1ul of GelRed into the mixture.
-
Pour the solution into a cast with an appropriate comb.
-
Leave to set
Running the Gel
- Mix 1ul of 1kbp DNA ladder with 6ul of loading dye (bromophenol blue) and 4ul of 1x TAE buffer (total 6ul) and load onto first well
- Mix 5ul of PCR products with 1ul of loading dye and load onto wells.
- Run gel at 90V for 45minutes approximately
- Photograph gels under UV light
- Mix 1g of agarose powder with 100ml of 1x TAE buffer and heat for 1minute or until all agarose is dissolved.
- Wait until it has cooled (not set), and add 1ul of GelRed into the mixture.
- Pour the solution into a cast with an appropriate comb.
- Leave to set
- Mix 1ul of 1kbp DNA ladder with 6ul of loading dye (bromophenol blue) and 4ul of 1x TAE buffer (total 6ul) and load onto first well
- Mix 5ul of PCR products with 1ul of loading dye and load onto wells.
- Run gel at 90V for 45minutes approximately
- Photograph gels under UV light
