From 2014.igem.org
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Revision as of 19:06, 3 July 2014
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Notebook |
Overview
Week 1
Week 2
Week 3
Week 4
Week 5
Week 6
Week 7
Week 8
Week 9
Week 10
Week 11
Week 12
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Week One |
Tuesday, 6/17/14
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- Get acquainted with summer logistics and lab basics
- Clean up lab space, stock lab supplies
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Wednesday, 6/18/14
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- Continue to discuss project and clean up lab space
- Designed PCR primers for combinatorial promoters subproject
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Thursday, 6/19/14
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- Formulated plasmid design (pAA001) for the response regulation subproject
- PCR run on plasmid backbone and sfGFP to create constructs with the proper overhangs for Gibson assembly
- Gel electrophoresis for confirmation of PCR products' length
- With the exception of the lacI gene, PCR products were confirmed via gel electrophoresis
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Friday, 6/20/14
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- Purification of PCR products created yesterday
- Gibson assembly of purified products
- Transformation of Gibson-assembled plasmids into JM109 E. coli
- Meeting with Prof. Murray to discuss give an update on the project
- Colonies were found and picked after transformation and incubation.
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