Team:Bielefeld-CeBiTec/Project/CO2-fixation/CalvinCycle

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<h6>Sedoheptulose 1,7-bisphosphatase</h6>
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     <p>The SBPase is one of enzymes needed for the Calvin cycle. It catalyzes the reaction from sedoheptulose 1,7-bisphosphate to sedoheptulose 7-phosphate. The enzyme is characteristic for the part of regeneration in the Calvin-cycle. It was shown before that oveerexpression of the SBPase in tobacco results in enhanced carbon assimilation and crop yield (<a href="rosenthal2011">Rosenthal et al., 2011</a>). SBPases are homodimeric with two identical subunits of 35kD to 38kD. The <i>K<sub>M</sub></i>-value of GlpX (<i>Bacillus methanolicus</i>) is 14 &plusmn; 0.5 µM (<a href="#stolzenberger2013">Stolzenberger et al., 2013</a>).<br> It does not occur in <i>E.coli</i> which makes it a target to transform for enabling the whole cycle.</p>
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     <p>The SBPase is one of enzymes needed for the Calvin cycle. It catalyzes the reaction from sedoheptulose 1,7-bisphosphate to sedoheptulose 7-phosphate. The enzyme is characteristic for the part of regeneration in the Calvin-cycle. It was shown before that oveerexpression of the SBPase in tobacco results in enhanced carbon assimilation and crop yield (<a href="rosenthal2011">Rosenthal et al., 2011</a>). SBPases are homodimeric with two identical subunits of 35kD to 38kD. The <i>K<sub>M</sub></i>-value of GlpX (<i>Bacillus methanolicus</i>) is 14 &plusmn; 0.5 µM (<a href="#stolzenberger2013">Stolzenberger et al., 2013</a>).<br> It does not occur in <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Organisms#E.coli" target="_blank><i>E.coli</i></a> which makes it a target to transform for enabling the whole cycle.</p>
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       Parikh et al., 2006. Directed evolution of RuBisCO hypermorphs through genetic selection in engineered <i>E.coli</i>. <a href="http://peds.oxfordjournals.org/content/19/3/113.long" target="_blank">Protein Engineering, Design & Selection</a>, vol. 19, pp. 113-119
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       Parikh et al., 2006. Directed evolution of RuBisCO hypermorphs through genetic selection in engineered <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Organisms#E.coli" target="_blank"><i>E.coli</i></a>. <a href="http://peds.oxfordjournals.org/content/19/3/113.long" target="_blank">Protein Engineering, Design & Selection</a>, vol. 19, pp. 113-119
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Revision as of 23:43, 15 October 2014


CO2 Fixation

Calvin cycle

The Calvin cycle (figure 1) is one of the most important pathways for carbon dioxide fixation in photoautotrophic organisms (Hügler and Sievert, 2011). Usually it uses the energy and reduction equivalents which are produced in the light reaction. This molecules could also be supplied from another source. The aim of our project is electroautotrophic growth of E. coli. Electric power replaces light as energy source of our Calvin cycle. Most of the enzymes needed in this pathway are already present in E. coli. This is due to the fact that this cycle could run in both directions. E. coli uses some parts of this pathway in another context. All in all we identified three reaction steps for which enzymes are missing. Sedoheptulose-1,7-bisphosphatase, phosphoribulokinase and RubisCO needs to be heterologous expressed. We will focus on this enzymes in the following parts.


Figure 1: Reactions of the Calvin cycle
Sedoheptulose 1,7-bisphosphatase

The SBPase is one of enzymes needed for the Calvin cycle. It catalyzes the reaction from sedoheptulose 1,7-bisphosphate to sedoheptulose 7-phosphate. The enzyme is characteristic for the part of regeneration in the Calvin-cycle. It was shown before that oveerexpression of the SBPase in tobacco results in enhanced carbon assimilation and crop yield (Rosenthal et al., 2011). SBPases are homodimeric with two identical subunits of 35kD to 38kD. The KM-value of GlpX (Bacillus methanolicus) is 14 ± 0.5 µM (Stolzenberger et al., 2013).
It does not occur in
Figure 2: Reaction of sedoheptulose 1,7-bisphosphatase

Phosphoribulokinase A

The phosphoribulokinase A is the enzyme which catalyzes the reaction from ribulose 5-phosphate to ribulose 1,5-bisphosphate. This step needs ATP.


Figure 3: Reaction of phosphoribulokinase
Ribulose 1,5-bisphosphate Carboxylase Oxygenase (RuBisCO)

The Ribulose 1,5-bisphosphate carboxylase oxygenase (RuBisCO) is the most abundant enzyme in the world. Because of its key role in carbon fixation metabolism, it is found in nearly all autotrophic organisms like plants, but also in cyanobacteria and fotosynthetic bacteria in high concentrations (Andersson, 2008). RuBisCo catalyses the fixation of atmospheric carbon dioxide by generating two tricarbohydrates out of one pentacarbohydrate. It can be stated that the RuBisCo is responsible for conversion of carbon dioxide in biomass or with other words for incorporation of inorganic carbon dioxide to form organic molecules. To give some numbers, more than 1011 tons of atmospheric carbon dioxide are fixated per year baesd on RuBisCo activity (Field et al., 1998).



Catalyzed reaction by the RuBisCo

RuBisCo catalyses the rate limiting step in the Calvin Cycle. The Calvin cycle is the light independent reaction of photosynthesis. In this cycle, carbon dioxide is fixed to build up energy-rich substrates. RuBisCo catalyses the fixation of one molecule carbon dioxide to ribulose-1,5-bisphosphate (RuBP), a pentacarbohydrate. The product is unstable and decays directly into two molecules of 3-D-phosphoglycerate (3-PGA)(Andersson, 2008) (Parikh et al. 2006). 3-PGA is further converted in the Calvin cycle to glycerinaldehyde-3-phosphate and metabolized by the cells. Furthermore 3-D-phosphoglycerate is an essential intermediate in the central metabolism, as it plays a central role in glycolysis and gluconeogenesis.
Beside the carbon fixation reaction of the RuBisCo, the enzyme catalyses numerous side reactions. An alternative substrate to carbon dioxide is atmospheric oxygen. When the oxygenation of RuBP ist catalyzed instead of carboxylation, the product is one molecule 3-PGA and one molecule 2-phosphoglycolate. 2-phosphoglycolate has only limited use for the metabolism of the cells and the fixed carbon has to be regenerated by a metabolic pathway called photorespiration, a high-energy consuming pathway. In photorespiration, two molecules 2-phosphoglycolate are split up in one molecule 3-PGA and one molecule carbon dioxide. 3-PGA can enter the Calvin cycle, whereas CO2 is unusable for the cells. Because of the oxygenation side reaction the effiziency of carbon dioxide fixation by RuBisCo is reduced about 20 - 50 % (Andersson, 2008) (Mann, 1999).
The catalyzed carboxylation/ oxygenation of RuBP is a multiple step reaction. In detail, the first step is activation of the RuBisCo by carbamylation of the amino group from a Lysin in the active centre. The activated RuBisCo is then stabilized by magnesium ions, a cofactor for activity. In the carboxylation/ oxygenation of RuBP the first step is enolisation of the substrate and enol-RuBP is build up. The enediolate reacts then in an irreversible, partial reaction with either carbon dioxide or oxygen. This reaction determines the specifity and the rate of carbon dioxide fixation as well as the effizenz. If carbon dioxide is bound by the enediolate, the instabile intermediate is protonated and hydrated to build up two molecules 3-PGA. If oxygen is bound by the enediolate, the intermediate decomposes directyl in 3-phosphoglycerate and 2-phosphoglycolate. (Andersson, 2008), (Spreitzer, Salvucci 2002)
The competing reaction between CO2 and O2 and the resulting oxygenation side reaction limits the efficiency of RuBisCo. The effiziency is often quantified by a specifity factor. This is the ration of the catalytic efficiency of carboxylation to oxygenation, described by the maximal velocities of carboxylation and oxygenation, and the Michaelis-Menten constants for carbon dioxide and oxygen. (Andersson, 2008) (Jordan, Ogren 1981) (Spreitzer, Salvucci 2002) The specifity factors of RuBisCo enzymes differ significantly depending on the host organism of the RuBisCo. Bacteria have low specifity factors in comparison to higher plants oder algae. As there exist a inverse correlation between turnover rate (for carboxylation) and specifity factor, Bacteria have low specifity factors, but high turnover rates. Higher organism are characterized by high specifity factors and low turnover rates. (Andersson, 2008) (Jordan, Ogren 1981)
Carboxysomes, bacterial microcompartiments, are one mechanism for carbon dioxide concentration. By expression of carboxysomes the carbon fixation efficiency is increased, using the high turnover rates. (Bonacci et al., 2011)
RuBisCo is a multiprotein enzyme, containing two types of subunits, the large (L) subunit (50-55 kDa) and the small (S) subunit (12-18 kDa). The most common form of RuBisCo (form I or form IA) consist of eight large subunits, which are forming dimers, and eight small subunits. Together they form a hexadimeric structure. Form I occurs in most autotrophic bacteria, algae and higher plants. The large subunit is the catalytic one, and the small subunit is not essential for catalysis. The octamer of large subunit still remains carboxylation activity. RuBisCo form II or form IB is found in some chemoautotrophic bacteria and in dinoflagellates. This form is characterized by the abscence of the small subunits. (Andersson, 2008) (Spreitzer, Salvucci 2002)

References

  • Andersson, 2008. Catalysis and regulation in Rubisco. Journal of Experimental Botany, vol. 59, pp. 1555-1568
  • Bonacci et al., 2011. Modularity of carbon-fixing protein organelle. PNAS, vol. 109, pp. 478-483
  • Jordan, Ogren 1981. Species variation in the specifity of ribulose biphosphate carboxylase/oxygenase. Nature, vol. 291, pp. 513-515
  • Mann, 1999. Genetic Engineers Aim to Soup up Crop Photosynthesis. Science, vol. 283, pp. 314-316
  • Parikh et al., 2006. Directed evolution of RuBisCO hypermorphs through genetic selection in engineered E.coli. Protein Engineering, Design & Selection, vol. 19, pp. 113-119
  • Rosenthal et al., 2011. Overexpressing the C(3) photosynthesis cycle enzyme sedoheptulose 1,7-bisphosphatase improves photosynthetic carbon gain and yield under fully open air CO(2) fumigation (FACE). BMC Plant Biol., vol. 11, pp. 123
  • Spreitzer, Salvucci, 2002. RUBISCO: Structure, Regulatory Interactions, and Possibilities for a Better Enzyme. Annu. Rev. Plant Biol., vol. 53, pp. 449-475
  • Stolzenberger et al., 2013. Characterization of Fructose 1,6-Bisphosphatase and Sedoheptulose 1,7-Bisphosphate from the Facultative Ribulose Monophosphate Cycle Methylotroph Bacillus methanolicus. Journal of Bacteriology, Vol. 195, pp. 5112-5122
  • Hügler, Michael, und Stefan M. Sievert. „Beyond the Calvin Cycle: Autotrophic Carbon Fixation in the Ocean“. Annual Review of Marine Science 3 (2011): 261–89. doi:10.1146/annurev-marine-120709-142712.