Team:Bielefeld-CeBiTec/Project/Isobutanol/Isobutanol

From 2014.igem.org

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   <h4>Production</h4>
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<p>In 1998 the U.S. EPA Inventory Update Report (IUR) listed 16 manufacturing facilities in the United States. These produced between 100 and 500 million pounds of isobutanol, which are 45.4 – 227.3 thousand metric tons. Manufacturing facilities of other regions or countries including their manufacturing capacities are listed in the following table (INCHEM, 2004).
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<p>In 1998 the U.S. EPA Inventory Update Report (IUR) listed 16 manufacturing facilities in the United States. These produced between 100 and 500 million pounds of isobutanol, which are 45.4 – 227.3 thousand metric tons. Manufacturing facilities of other regions or countries including their manufacturing capacities are listed in the following table (<a href="#INCHEM2004">INCHEM, 2004</a>).
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As the table shows there are 3 big markets for isobutanol in the United States. The largest one is the production of zinc dialkyldithiophosphates (ZDDP). ZDDP is an additive for lube oils, greases and hydraulic fluids, which work as anti-wear and corrosion inhibitors. <br>
As the table shows there are 3 big markets for isobutanol in the United States. The largest one is the production of zinc dialkyldithiophosphates (ZDDP). ZDDP is an additive for lube oils, greases and hydraulic fluids, which work as anti-wear and corrosion inhibitors. <br>
The conversion of isobutanol to isobutyl acetate is the second largest market.<br>
The conversion of isobutanol to isobutyl acetate is the second largest market.<br>
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The use of isobutanol as a solvent is the third largest market. It is mainly used for surface coatings and adhesives. Hence it is used for as a latent solvent in surface coatings or even as a processing solvent in the production of e.g. pesticides and pharmaceuticals (INCHEM, 2004).<br>
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The use of isobutanol as a solvent is the third largest market. It is mainly used for surface coatings and adhesives. Hence it is used for as a latent solvent in surface coatings or even as a processing solvent in the production of e.g. pesticides and pharmaceuticals (<a href="#INCHEM2004">INCHEM, 2004</a>).<br>
All in all isobutanol is an important substance for industrial use and large amounts are needed all over the world.
All in all isobutanol is an important substance for industrial use and large amounts are needed all over the world.
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INCHEM: <a href="http://www.inchem.org/documents/sids/sids/78831.pdf" target="_blank">SIDS Initial Assessment Report
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For SIAM 19 </a>, version: 15/10/2014
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Revision as of 20:58, 15 October 2014


Isobutanol

Product Synthesis

The third module benefits from the two modules before. E. coli has gained energy equivalents and reduction equivalents to generate pyruvate by binding carbon dioxide. By using the Calvin cycle the product of our CO2 fixation is pyruvate. This substance is now used as the initial point for the product synthesis. Pyruvate is a starting point of the producing pathways of a variety of high value products like isobutanol, isoprene, putrescine or even antibiotics. Our project implements the producing pathway of isobutanol by using and improving existing BioBricks (iGEM Team Formosa 2011/2012).


Figure 1: Schematic illustration of module III
The product synthesis can be changed through the modularity of BioBricks so that variable high value products derived from pyruvate can be implemented in the future.

Isobutanol

Isobutanol is an amino-acid-based alcohol which is an organic substance.

It can be produced by the 2-keto-acid, or Ehrlich, pathway. Keto acids, the immediate amino-acid precursors, are decarboxylated into aldehydes and reduced to alcohols. By using this pathway amino-acid-based alcohols can be produced. These include n-butanol from norvaline, n-propanol from isoleucineand isobutanol from valine. Although the energy contents of isobutanol and n-butanol are similar, isobutanol is the closest to industrial use. Because of its branching it has improved properties, like a better octane number. This number is nessesarry to measure a fuel's resistance to knocking in spark ignition engines. (Pamela P. Peralta-Yahya et al., 2012)
In the following table you can find some general information about isobutanol.

CAS Number78-83-1
IUPAC Name2-methyl-propan-1-ol
Synonymsisobutyl alcohol
IBA, IBOH
fermentation butyl alcohol
1-hydroxymethylpropane
isobutanol
isopropylcarbinol
2-methylpropanol
2-methyl-1-propanol
2-methylpropan-1-ol
2-methylpropyl alcohol
Molecular FormulaC4H10O
Structural Formula(CH3)2-CH-CH2OH
Molecular Weight74.12 g/mol
Physical stateLiquid
Melting point-108°C
Boiling point108°C
Water solubility85.0 g/l at 25°C



Production

In 1998 the U.S. EPA Inventory Update Report (IUR) listed 16 manufacturing facilities in the United States. These produced between 100 and 500 million pounds of isobutanol, which are 45.4 – 227.3 thousand metric tons. Manufacturing facilities of other regions or countries including their manufacturing capacities are listed in the following table (INCHEM, 2004).

Region or countryNumber of producersManufacturing capacities [metric tons]
Western Europe4160,000
Eastern Europe369,000 (including some n-butyl alcohol)
Russia348,000
Iran16,000
Japan343,000
China214,000
Indian.a.8,000 (including some n-butyl alcohol)
Indonesia110,000
Korea225,000
Brazil119,000



Use

Isobutanol has many applications. In the following table you can find a list of uses and how many isobutanol is applied for the various uses in the United States

ApplicationAmount
lube oil additives19 thousand metric tons
(in which isobutyl alcohol is an intermediate
to produce the lube oil additive ZDDP)
conversion to isobutyl acetate10 thousand metric tons
direct solvent9 thousand metric tons
conversion to amino resins7 thousand metric tons
conversion to isobutylamines1 thousand metric tons
conversion to acrylate and methacrylate esters1 thousand metric tons
other uses1 thousand metric tons
All in all 47 thousand metric tons produced in the United States

As the table shows there are 3 big markets for isobutanol in the United States. The largest one is the production of zinc dialkyldithiophosphates (ZDDP). ZDDP is an additive for lube oils, greases and hydraulic fluids, which work as anti-wear and corrosion inhibitors.
The conversion of isobutanol to isobutyl acetate is the second largest market.
The use of isobutanol as a solvent is the third largest market. It is mainly used for surface coatings and adhesives. Hence it is used for as a latent solvent in surface coatings or even as a processing solvent in the production of e.g. pesticides and pharmaceuticals (INCHEM, 2004).
All in all isobutanol is an important substance for industrial use and large amounts are needed all over the world.

References