Team:UC Davis/Electrochemistry

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We developed our Electrode System to be:
We developed our Electrode System to be:
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Revision as of 20:46, 15 October 2014

UC Davis iGEM 2014

Electrode Choice

Electrode Choice

System Optimization

System Optimization

Coupling Enzymes

Coupling Enzymes

Having settled on NAD+ dependent Aldehyde Dehydrogenases as our method of differentiating between aldehydes, we needed to develop an efficient electrode system to detect enzyme activity via NADH. We acquired, selected, and optimized an electrode setup for the detection of NADH at low concentrations in a complex solution. Additionally, we demonstrated the ability of the electrode setup to detect enzyme generated NADH over time, and thereby functionally deconvolute aldehyde profiles within a sample.

Electrode Choice

We developed our Electrode System to be:

  • Sensitive: have a low limit of detection for NADH
  • Reactive: Detect NADH with high linear range
  • Selective: Be robust to any possible solution components
  • Affordable: Cost accessible to the average consumer
  • Efficient: Use a low sample volume
  • Compatible: Be compatible with our, as well as other potentiostats
  • Portable


We tested three screen printed base electrode types, and five different working electrode modification schemes in order to achieve the requisite sensitivity for our system. We settled on Dropsens screen printed #610 Electrodes, depicted above. To find out more about our electrode selection process, click here.

System Optimization

Here is some sample text

Coupling Enzymes

Here is some sample text