Team:ETH Zurich/lab/overview
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Before combining all the aspects of our project we divided it into several experimental subunits first to answer our crucial questions: | Before combining all the aspects of our project we divided it into several experimental subunits first to answer our crucial questions: | ||
- | * Can we produce a millifluidic PDMS chip with a 3D-printed mold? | + | * Can we produce a [https://2014.igem.org/Team:ETH_Zurich/lab/chip millifluidic PDMS chip] with a 3D-printed mold? |
- | * Do ''E. coli'' cells still grow and produce fluorescent proteins and signal molecules when encapsulated into alginate beads? | + | * Do ''E. coli'' cells still grow and produce fluorescent proteins and signal molecules when [https://2014.igem.org/Team:ETH_Zurich/lab/bead encapsulated into alginate beads]? |
- | * | + | * Can the signal [https://2014.igem.org/Team:ETH_Zurich/expresults#Diffusion_On_Chip diffuse through the channels] of the millifluidic chip and activate the cells in the neighboring bead? |
- | Click on the different subunits to learn more about how we worked in the lab and what our [https://2014.igem.org/Team:ETH_Zurich/expresults results] were. | + | * Is there crosstalk between the different [https://2014.igem.org/Team:ETH_Zurich/expresults#Quorum_Sensing quorum sensing] systems? |
+ | * Do [https://2014.igem.org/Team:ETH_Zurich/expresults#Riboregulators riboregulators] decrease the problem of leakiness? | ||
+ | * Are the [https://2014.igem.org/Team:ETH_Zurich/expresults#Integrases integrase] based logic gates working in our setup? | ||
+ | '''Click on the different subunits''' to learn more about how we worked in the lab and what our [https://2014.igem.org/Team:ETH_Zurich/expresults results] were. | ||
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Latest revision as of 15:23, 15 October 2014
Lab overview
- Can we produce a millifluidic PDMS chip with a 3D-printed mold?
- Do E. coli cells still grow and produce fluorescent proteins and signal molecules when encapsulated into alginate beads?
- Can the signal diffuse through the channels of the millifluidic chip and activate the cells in the neighboring bead?
- Is there crosstalk between the different quorum sensing systems?
- Do riboregulators decrease the problem of leakiness?
- Are the integrase based logic gates working in our setup?
Click on the different subunits to learn more about how we worked in the lab and what our results were.
![](https://static.igem.org/mediawiki/2014/c/ce/ETH_Zurich_2014_Lab_overview_new.png)