Team:Bielefeld-CeBiTec/Notebook/Journal/Isobutanol/Sep

From 2014.igem.org

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<li><b><i>pSB1C3_<i>alsS</i>_<i>ilvC</i>_<i>ilvD</i>_<i>kivD</i>_<i>adhA</i></i></b></li>
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<li>This week we tried to contruct the pSB1C3_<i>alsS</i>_<i>ilvC</i>_<i>ilvD</i>_<i>kivD</i>_<i>adhA</i>-Plasmid by <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#NEBBioBrick" target="_blank">NEB BioBrick Assembly</a></li>
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                      <ul>
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<li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#NEBBioBrick" target="_blank">NEB BioBrick Assembly</a></li>
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<li>Upstream part pSB1K3_<i>alsS</i>_<i>ilvC</i>_<i>ilvD</i>_<i>kivD</i> (digested with <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#RestrictionDigestion" target="_blank"><i>EcoR</i>I</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#RestrictionDigestion" target="_blank"><i>Spe</i>I</a>)</li>
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<li><i>alsS</i>_<i>ilvC</i>_<i>ilvD</i>_<i>kivD</i></li>
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<li>Downstream part pSB1K3_<i>adhA</i>(digested with <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#RestrictionDigestion" target="_blank"><i>Xba</i>I</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#RestrictionDigestion" target="_blank"><i>Pst</i>I</a>)</li>
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<li><i>adhA</i></li>
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                                  <li>Destination part pSB1C3_RFP (digested with <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#RestrictionDigestion" target="_blank"><i>EcoR</i>I</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#RestrictionDigestion" target="_blank"><i>Pst</i>I</a>)</li>
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<ul>
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<li><i>alsS</i>_<i>ilvC</i>_<i>ilvD</i>_<i>kivD</i></li>
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</ul>
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Revision as of 15:22, 15 October 2014


September

  • T7_alsS_ilvC_ilvD_kivD
    • This week we tried to verify the positive cutures that were identified last week by a restriction digest
  • pSB1K3_alsS_ilvC_ilcD_kivD_adhA

  • pSB1C3_alsS_ilvC_ilvD_kivD
    • pSB1C3_alsS_ilvC_ilvD_kivD_adhA
      • This week we tried to contruct the pSB1C3_alsS_ilvC_ilvD_kivD_adhA-Plasmid by NEB BioBrick Assembly
        • NEB BioBrick Assembly
          • Upstream part pSB1K3_alsS_ilvC_ilvD_kivD (digested with EcoRI, SpeI)
            • alsS_ilvC_ilvD_kivD
          • Downstream part pSB1K3_adhA(digested with XbaI, PstI)
            • adhA
          • Destination part pSB1C3_RFP (digested with EcoRI, PstI)
            • alsS_ilvC_ilvD_kivD