Team:Bielefeld-CeBiTec/Notebook/Journal/Isobutanol/Sep

From 2014.igem.org

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<ul>
<ul>
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<li><b><i>pSB1K3_<i>alsS</i>_<i>ilvC</i>_<i>ilvD</i>_<i>kivD</i></i></b></li>
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<li><b><i>pSB1C3_<i>alsS</i>_<i>ilvC</i>_<i>ilvD</i>_<i>kivD</i></i></b></li>
<ul>
<ul>
<li>This week we tried to reclone <i>alsS</i>_<i>ilvC</i>_<i>ilvD</i>_<i>kivD</i> into pSB1C3 </li>
<li>This week we tried to reclone <i>alsS</i>_<i>ilvC</i>_<i>ilvD</i>_<i>kivD</i> into pSB1C3 </li>
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<li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Transformationviaelectroporation" target="_blank">Transformation</a> with electrocompotetent cells</li>   
<li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Transformationviaelectroporation" target="_blank">Transformation</a> with electrocompotetent cells</li>   
</ul>
</ul>
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</ul>  
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<ul>
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<li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#ColonyPCR" target="_blank">Colony PCR</a> (<a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#fw_ilvD_kivD" target="_blank">fw_ilvD_kivD</a>, <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#rv_pSB1C3_kivD" target="_blank">rv_pSB1C3_kivD</a>)
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            </li>
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<ul>
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<li>Annealing temperature: 65 °C</li>
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<li>Bands not as expected (~1800 bp)</li>
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</ul>
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</ul>
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</ul>
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<li>bla</li>
</ul>  
</ul>  
<br>
<br>

Revision as of 14:37, 15 October 2014


September

  • T7_alsS_ilvC_ilvD_kivD
    • This week we tried to verify the positive cutures that were identified last week by a restriction digest
  • pSB1K3_alsS_ilvC_ilcD_kivD_adhA