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Team:Bielefeld-CeBiTec/Notebook/Journal/Isobutanol/Sep
From 2014.igem.org
(Difference between revisions)
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<ul><li>This week we tried to verify the positive cutures that were identified last week by a restriction digest</li> | <ul><li>This week we tried to verify the positive cutures that were identified last week by a restriction digest</li> | ||
| + | <ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of pSB1K3_<i>adhA</i></li> | ||
| + | </ul> | ||
<ul> | <ul> | ||
| Line 62: | Line 65: | ||
</div> | </div> | ||
| - | </ul> </ul></ul></ul> | + | </ul> </ul><li>Successful sequencing</li></ul></ul><ul> |
| + | <li><b><i>T7_alsS_ilvC_ilvD_kivD</i></b></li> | ||
| + | <ul><li>This week we tried to verify the positive cutures that were identified last week by a restriction digest</li> | ||
| + | |||
| + | <ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#PurificationPromega" target="_blank">Plasmid isolation</a> of pSB1A2_T7_<i>alsS</i>_<i>ilvC</i>_<i>ilvD</i>_<i>kivD</i></li> | ||
| + | </ul> | ||
| + | <ul> | ||
| + | <li><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#RestrictionDigestion" target="_blank">Restriction digestion</a> with <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#RestrictionEnzymes" target="_blank"><i>EcoR</i>I</a> and <a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/KitsAndEnzymes#RestrictionEnzymes" target="_blank"><i>Pst</i>I</a> of pSB1A2_T7_<i>alsS</i>_<i>ilvC</i>_<i>ilvD</i>_<i>kivD</i> </li> | ||
| + | <ul> | ||
| + | <li>Bands not as expected (~7200bp)</li> | ||
| + | </ul> </ul></ul></ul></ul> | ||
</div> | </div> | ||
</div> | </div> | ||
Revision as of 09:03, 15 October 2014
 |
September |
 |
- adhA
- This week we tried to verify the positive cutures that were identified last week by a restriction digest
- Plasmid isolation of pSB1K3_adhA
- Restriction digestion with EcoRI and PstI of pSB1K3_adhA
- Bands as expected (~1200 bp)
- Successful sequencing
Agarose gel from the restriction digestion with EcoRI and PstI. As a Ladder we used GeneRuler™ 1 kb DNA Ladder from Thermo Scientific.
- T7_alsS_ilvC_ilvD_kivD
- This week we tried to verify the positive cutures that were identified last week by a restriction digest
- Plasmid isolation of pSB1A2_T7_alsS_ilvC_ilvD_kivD
- Restriction digestion with EcoRI and PstI of pSB1A2_T7_alsS_ilvC_ilvD_kivD
- Bands not as expected (~7200bp)
