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Team:GeorgiaTech/Parts
From 2014.igem.org
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<tr><td>BBa_K1539008<td>Part<td>This coding sequence is for the gene sMMO B, encoding Protein B of the multi-complex enzyme soluble methane monooxyegnase (sMMO). The gene has been codon optimized for E. coli.<td>426 bp | <tr><td>BBa_K1539008<td>Part<td>This coding sequence is for the gene sMMO B, encoding Protein B of the multi-complex enzyme soluble methane monooxyegnase (sMMO). The gene has been codon optimized for E. coli.<td>426 bp | ||
<tr><td>BBa_K1539013<td>Part<td>This coding sequence is for the gene sMMO C, encoding Protein C of the multi-complex enzyme soluble methane monooxyegnase (sMMO). The gene has been codon optimized for E. coli.<td>1047 bp | <tr><td>BBa_K1539013<td>Part<td>This coding sequence is for the gene sMMO C, encoding Protein C of the multi-complex enzyme soluble methane monooxyegnase (sMMO). The gene has been codon optimized for E. coli.<td>1047 bp | ||
| - | <tr><td>BBa_K1539021<td>Device<td>This primer | + | <tr><td>BBa_K1539021<td>Device<td>This primer is used to insert a high efficiency RBS to transcribe genes starting with ATG in E. coli.<td>46 bp |
| - | <tr><td>BBa_K1539034<td>Device<td>This primer | + | <tr><td>BBa_K1539034<td>Device<td>This primer is used to insert a low efficiency RBS to transcribe genes starting with ATG in E. coli.<td>46 bp |
| - | <tr><td>BBa_K1539055<td>Device<td>This primer | + | <tr><td>BBa_K1539055<td>Device<td>This primer is used to insert a high efficiency promoter to translate E. coli. genes. Must be used after insertion of RBS primer. Use Georgia Tech iGEM RBS primers (BBa_K1539021 or BBa_K1539034) or an RBS primer with a similar design.<td>58 bp |
| - | <tr><td>BBa_K1539089<td>Device<td>This primer | + | <tr><td>BBa_K1539089<td>Device<td>This primer is used to insert a low efficiency promoter to translate E. coli. genes. Must be used after insertion of RBS primer. Use Georgia Tech iGEM RBS primers (BBa_K1539021 or BBa_K1539034) or an RBS primer with a similar design.<td>58 bp |
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Revision as of 05:22, 15 October 2014
Biobricks and Devices
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K1539001 | Part | Coding sequence has a high efficiency promoter followed by a high efficiency RBS to express fluorescent protein mCherry. | 814 bp |
| BBa_K1539002 | Part | Coding sequence has a low efficiency promoter followed by a low efficiency RBS to express fluorescent protein mCherry. | 814 bp |
| BBa_K1539003 | Part | This coding sequence is for the beta subunit for Protein A (composed of three subunits) of the multi-complex enzyme soluble methane monooxyegnase (sMMO). The gene has been codon optimized for E. coli. | 1170 bp |
| BBa_K1539005 | Part | This coding sequence is for the gamma subunit for Protein A (composed of three subunits) of the multi-complex enzyme soluble methane monooxyegnase (sMMO). The gene has been codon optimized for E. coli. | 513 bp |
| BBa_K1539008 | Part | This coding sequence is for the gene sMMO B, encoding Protein B of the multi-complex enzyme soluble methane monooxyegnase (sMMO). The gene has been codon optimized for E. coli. | 426 bp |
| BBa_K1539013 | Part | This coding sequence is for the gene sMMO C, encoding Protein C of the multi-complex enzyme soluble methane monooxyegnase (sMMO). The gene has been codon optimized for E. coli. | 1047 bp |
| BBa_K1539021 | Device | This primer is used to insert a high efficiency RBS to transcribe genes starting with ATG in E. coli. | 46 bp |
| BBa_K1539034 | Device | This primer is used to insert a low efficiency RBS to transcribe genes starting with ATG in E. coli. | 46 bp |
| BBa_K1539055 | Device | This primer is used to insert a high efficiency promoter to translate E. coli. genes. Must be used after insertion of RBS primer. Use Georgia Tech iGEM RBS primers (BBa_K1539021 or BBa_K1539034) or an RBS primer with a similar design. | 58 bp |
| BBa_K1539089 | Device | This primer is used to insert a low efficiency promoter to translate E. coli. genes. Must be used after insertion of RBS primer. Use Georgia Tech iGEM RBS primers (BBa_K1539021 or BBa_K1539034) or an RBS primer with a similar design. | 58 bp |
