Team:UCL

From 2014.igem.org

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                <li> <a href="/Team:UCL/about">  
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    <h2>WHO ARE WE?</h2>
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    <p>We are University College London's iGEM 2014 team.</p>
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                    <img src="https://static.igem.org/mediawiki/2014/b/b3/PN_gbad_350x300.png" alt="" /> </a>  
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    <h2>WHAT ARE WE DOING?</h2>
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    <p>We are using Synthetic Biology to combat <b>AZO-DYE</b> Pollution.  Azo Dyes are the most common synthetic dye and are used everywhere, from food colouring to clothes. You're probably wearing clothes dyed with Azo-Dyes! But, when they get into waste water they're very toxic. Our team is engineering bacteria that degrade these dyes, using enzymes, to make them colourless and less harmful to the environment</p>
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<br/><br/><p>FIND OUT MORE</p>
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                        <a href="/Team:UCL/about">  
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                        <h3>About our Project</h3>
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                        <p>Everything there is to know about the science and logistics behind our journey.</p>
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<div class="box" id="biobricks-pro"><a href="https://2014.igem.org/Team:UCL/Project/Biobricks"><span class="overlayx"></span></a></div>
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                <li> <a href="/Team:UCL/team_ug"> <img src="https://static.igem.org/mediawiki/2014/8/83/Group_social_350x450.png" alt="" /></a>
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                        <a href="/Team:UCL/team_ug">
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                        <h3>Meet the team</h3>
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                        <p>Get to know us! Learn of all our diverse background and how we are all making this iGEM team brilliant. <br>(And that we're not just a team of guys!)</p>
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                <li> <a href="/Team:UCL/blog"> <img src="https://static.igem.org/mediawiki/2014/e/ef/EngOpenDay_EG_SJ.png" alt="" /></a>
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                    <div class="summary">  
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                        <a href="/Team:UCL/blog">
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                        <h3>Read our blog</h3>
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                        <p>The place where all of our soon to come blogs will reside.</p>
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                <li> <a href="/Team:UCL/gallery"> <img src="https://static.igem.org/mediawiki/2014/2/25/Natural-dyes_350x450.png" alt="" /></a>
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                        <h3>Gallery</h3>
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                        <p>A collection of all of the photos and pics we have, and are taking, in this great journey.</p>
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                        <h3>Frequently asked questions</h3>
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                        <p>Have no idea what we're talking about? Head on over here to have all your questions answered.</p>
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                        <h3>Contact us</h3>
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                        <p>Have a question or just want to chat? Fire off a message from here.</p>
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        <h2>IN THE LAB</h2>
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        <p>Some text about our lab work here</p>
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<br/><br/><p>FIND OUT MORE</p>
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<div class="box" id="results-sci"><a href="https://2014.igem.org/Team:UCL/Science/Results"><span class="overlayx"></span></a></div>
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        <div class="box" id="micro-faq"><a href="https://2014.igem.org/Team:UCL/Science/MicroF"><span class="overlayx"></span></a></div>
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        <h3 class="short_headline"><span>University College London, iGEM Team 2014 - Goodbye Azo Dye!</span></h3>
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        <h4>The World Problem: </h4>
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        <p><a href="/Team:UCL/about">Azo dyes</a> are the main synthetic chemical colourant used in the industrial manufacture of a wide range of products, such as clothing, cosmetics, tattoo ink, food, and more. These compounds are generally considered to produce safe and stable forms of dyes, which is why dye companies predominantly use azo dyes to colour their products. However, in some (textiles/dye) industries, leftover dye effluent is often not adequately disposed or removed during water treatment, which can result in the accumulation of <a href="/Team:UCL/risk">azo dyes in water bodies</a>. It is at this point that azo dyes can be ingested, broken down, and excreted as potentially toxic (mutagenic or carcinogenic) aromatic amine products. Removing azo dyes in a sustainable manner remains a major challenge, both for the developing world and industrialised nationals.
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        <h4>Our Synthetic Biology Solution: </h4>
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        <p>In response to this challenge, the project for the <a href="/Team:UCL/team_ug">UCL iGEM Team 2014</a> is to design, model, and construct an engineered Azo-Remediation Chassis (ARC) with <a href="/Team:UCL/biobricks">BioBrick Parts</a> that are:
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            <br>a) capable of sensing environmetal levels of azo compounds/dyes;
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            <br>b) efficent at degrading, and decolourising, these azo compounds;
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            <br>c) and able to utilise the azo dye breakdown products as substrates to build high-value fine chemicals. Welcome to project, "Goodbye Azo Dye"!</p>
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            <li>capable of sensing environmental levels of azo compounds/dyes; </li>
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            <li>efficient at degrading, and decolourising, these azo compounds; </li>
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            <li>and able to utilise the azo dye breakdown products as substrates to build high-value fine chemicals.
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            <br>Welcome to project, "Goodbye Azo Dye"!</li>
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        <h4>The General Approach: </h4>
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        <p>To validate the above approach, we will first introduce genes encoding three sequentially-acting enzymes (azoreductase, laccase, and lignin peroxidase) into a host <em>E. coli</em> cell. These transformed <em>E. coli</em> cells will then be introduced to a mixture of azo dyes (emulating wastewater).
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            <br>Stage 1: Azoreductase will cleave the azo bond (N=N) by a double reduction, using NAD(P)H as a cofactor; a series of highly toxic aromatic amines will be produced.
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            <br>Stage 2: The breakdown products of Stage 1 will then be oxidised by laccase and/or lignin peroxidase, which will complete the azo dye degradation, as well as result in a decolourisation of the azo dye solution. </p>
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            <li>Stage 1: Azoreductase will cleave the azo bond (N=N) by a double reduction, using NAD(P)H as a cofactor; a series of highly toxic aromatic amines will be produced. </li>
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            <li>Stage 2: The breakdown products of Stage 1 will then be oxidised by laccase and/or lignin peroxidase, which will complete the azo dye degradation, as well as result in a decolourisation of the azo dye solution. </li>
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        <p>This sequential degradation process should result in a decreased toxicity level of the azo dye mixture, to the point that the final products of this process are less toxic than the intact dyes themselves. The complementary action of the azoreductase and laccase and/or lignin peroxidase will be studied, both experimentally and through modelling, in order to find the best possible sequential approach. </p>
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        <p>Finally, this core degradation module will be extrapolated to other areas, such as BioArt projects and work on algal-bacterial symbiosis, to try set up the foundations for synthetic ecology. </p>
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            <div class="span4"> <a href="/Team:UCL/about"><img src="https://static.igem.org/mediawiki/2014/9/9e/About_banner.png" class="aligncenter" alt="" height=100 /></a>
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                <h3><a href="/Team:UCL/about">About our project</a></h3>
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                <p>Find out more about the issues of azo dyes, and how we are using synthetic biology to try solve this problem.</p>
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                <p class="right"><a href="/Team:UCL/about" class="btn btn-primary">Details &rarr;</a></p>
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                <h3><a href="/Team:UCL/team_ug">Meet the team</a></h3>
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                <p>Get to know us! Come find out our diverse and colourful backgrounds, and how we are all going to make this iGEM team brilliant.</p>
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                <p class="right"><a href="/Team:UCL/team_ug" class="btn btn-primary">Details &rarr;</a></p>
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                <h3><a href="/Team:UCL/blog">Read our blog</a></h3>
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                <p>See what we get up to throughout our project! <br><br><br> </p>
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                <p class="right"><a href="/Team:UCL/blog" class="btn btn-primary">Details &rarr;</a></p>
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        <h3 class="short_headline"><span>Latest updates</span></h3>
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                <h3><a href="/Team:UCL/faq">What is iGEM?</a></h3>
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                <p>More about the International Genetically Engineered Machine competition. <br><br> </p>
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                <p class="right"><a href="/Team:UCL/faq" class="btn btn-primary">Details &rarr;</a></p>
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                <h3><a href="/Team:UCL/synbio">New to Synthetic Biology?</a></h3>
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                <p>Find out more about the exciting new field of synthetic biology! <br><br><br> </p>
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                <p class="right"><a href="/Team:UCL/synbio" class="btn btn-primary">Details &rarr;</a></p>
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                <h3><a href="/Team:UCL/microfluids">The Basics to Microfluidics</a></h3>
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                <p>We'll also be applying the multidisciplinary field of microfluidics to our project. Find out more here!</p>
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                <p class="right"><a href="/Team:UCL/microfluids" class="btn btn-primary">Details &rarr;</a></p>
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                <h3><a href="/Team:UCL/biobricks">BioBrick Parts</a></h3>
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                <p>See what BioBrick parts we'll be creating and characterising to carry out our project. <br><br> </p>
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                <p class="right"><a href="/Team:UCL/biobricks" class="btn btn-primary">Details &rarr;</a></p>
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            <h3 class="short_headline margin-top"><span>Our sponsors</span></h3>
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                <li><img src="https://static.igem.org/mediawiki/2014/7/74/BBSRC_logo_2.png" alt="" /></li>
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                <li><img src="https://static.igem.org/mediawiki/2014/d/d7/BioChem_Soc_logo_2.png" alt="" /></li>
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                <li><img src="https://static.igem.org/mediawiki/2014/e/ee/MicroBio_logo_2.png" alt="" /></li>
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                <li><img src="https://static.igem.org/mediawiki/2014/4/43/Wellcome_Trust_logo_2.png" alt="" /></li>
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                <li><img src="https://static.igem.org/mediawiki/2014/1/1a/UCL_Eng_logo_2.png" alt="" /></li>
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    <h2>OUR STORY</h2>
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        <p>Humans are the engine behind the machine that is our project, and it is through humans that we tell our story. Be it who we are or the who we've influenced this is where you will find anything and everything to do with the humans involved in this project. Click <a href="https://2014.igem.org/Team:UCL/Humans/Story">here</a> to see the full story how we as a team engaged the public and ourselves, or click one of the boxes to see some of the more individual aspects of the humans in our project. 
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<br/><br/><p>FIND OUT MORE</p>
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Revision as of 20:26, 14 October 2014

Goodbye Azodye UCL iGEM 2014

WHO ARE WE?

We are University College London's iGEM 2014 team.

WHAT ARE WE DOING?

We are using Synthetic Biology to combat AZO-DYE Pollution. Azo Dyes are the most common synthetic dye and are used everywhere, from food colouring to clothes. You're probably wearing clothes dyed with Azo-Dyes! But, when they get into waste water they're very toxic. Our team is engineering bacteria that degrade these dyes, using enzymes, to make them colourless and less harmful to the environment



FIND OUT MORE

OUR STORY

Humans are the engine behind the machine that is our project, and it is through humans that we tell our story. Be it who we are or the who we've influenced this is where you will find anything and everything to do with the humans involved in this project. Click here to see the full story how we as a team engaged the public and ourselves, or click one of the boxes to see some of the more individual aspects of the humans in our project.



FIND OUT MORE

Contact Us

University College London
Gower Street - London
WC1E 6BT
Biochemical Engineering Department
Phone: +44 (0)20 7679 2000
Email: ucligem2014@gmail.com

Follow Us

Retrieved from "http://2014.igem.org/Team:UCL"