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<textarea cols="100" rows="8" id="learned_in_training" data-form-field="learned_in_training">Training in School safety procedures; training in good microbial and general laboratory practices. Familiarity with risk assessments including but not limited to GM, microbiology and those for specific techniques such as agarose gel electrophoresis.

<textarea cols="100" rows="4" id="inst_training_requirements" data-form-field="inst_training_requirements">The University of East Anglia (UEA) provides general safety advice and guidance, with specific details focusing on laboratory experiments. Specific information is provided on the UEA Intranet site, but an overview of general health and safety practice is available at: https://www.uea.ac.uk/uss</textarea>

<textarea cols="100" rows="8" id="inst_biosafety_officer" data-form-field="inst_biosafety_officer">The University of East Anglia (UEA) has several staff who have roles that are dedicated to dealing with all health and safety services at the institution. The Faculty of Sciences at UEA has a designated Safety Committee, which is manned by a range of administrative and academic staff who have a a specific focus on their duties. The School of Biological Sciences at UEA has a senior member of academic staff that oversees safety procedures for microbiological experiments and another who focuses on Genetic Modification studies. We have had discussions about our project with these various staff members and they are content that appropriate safety procedures are in place.</textarea>

<textarea cols="100" rows="5" id="inst_biosafety_guidelines" data-form-field="inst_biosafety_guidelines">The University of East Anglia (UEA) provides biosafety guidelines, with specific information provided on the UEA Intranet site at:  

<textarea cols="100" rows="10" id="lab_worker_risks_now" data-form-field="lab_worker_risks_now">Transmission to laboratory personnel is a potential hazard although there is no recorded precedence with the Escherichia coli strains that we will use.

<textarea cols="100" rows="5" id="public_risks_now" data-form-field="public_risks_now">The E. coli and A. tumefaciens hosts to be used have unknown colonising potential but have no recorded pathogenicity. The laboratory strains used are likely to be less fit to survive in the environment than wild type strains meaning that the potential risk of harm or infection to the general public is reduced. In the event of accidental release into the environment, no realistic implications are predicted.</textarea>

<textarea cols="100" rows="8" id="env_risks_now" data-form-field="env_risks_now">The ability of the E. coli and A. tumefaciens strains to survive in the environment is unknown. However, it is likely that the laboratory strains used are not as fit to survive in the environment compared to wild-type strains and therefore pose less of a risk.  

<textarea cols="100" rows="4" id="security_now" data-form-field="security_now">There are no known serious implications accociated with accidental release of the E. coli and A. tumefaciens strains as the laboratory strains used are less fit than wild-type strains or have no known pathogenicity. There is no forseeable malicious use for these strains that could cause a security threat.  </textarea>

<textarea cols="100" rows="14" id="reduce_risks_now" data-form-field="reduce_risks_now">Good laboratory practice and implementation will minimise risks. This will include restricted access to authorised workers, ensuring that all areas are swabbed with a recommended disinfectant, no laboratory clothing allowed outside of the laboratory and all outdoor clothing will be stored in offices. All contaminated waste will be autoclaved.  

<textarea cols="100" rows="4" id="future_risks" data-form-field="future_risks">Transmission of DNA into neighbouring crops could cause a problem regarding contamination of the food supply.  However, if we were to apply for a field-testing licence then our genetically modified plant biosensor would be prevented from reproducing and therefore would cause no forseeable problems in regards to transmission to neighbouring crops.</textarea>

<textarea cols="100" rows="2" id="minimize_future_risks" data-form-field="minimize_future_risks">If the project reaches the stage of being tested in the field, the plants would be prevented from reproducing to prevent gene transfer to neighbouring plants.</textarea>

<textarea cols="100" rows="3" id="further_comments" data-form-field="further_comments">Level 1 constructs have been made using golden gate cloning and transformed into E. coli. These are now ready to be transformed into Agrobacterium tumefaciens GV3101 for use in in transient expression experiments in Nicotiana Benthamiana.</textarea>