Team:Aachen/Notebook/Protocols/detection
From 2014.igem.org
(Difference between revisions)
m (→2D detection of IPTG and HSL) |
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</html> | </html> | ||
- | = 2D | + | = 2D Detection of IPTG and HSL = |
- | == Chip | + | == Chip Production == |
- | === Cell | + | === Cell Preparation === |
# over night culture of sensor cells (50 mL in a 250 mL flask with) max. 16 h | # over night culture of sensor cells (50 mL in a 250 mL flask with) max. 16 h | ||
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- | === Agar | + | === Agar Preparation === |
# autoclave 50 mL medium with 1.5 % (w/v) agarose (has to be multiplied with the number of chips prepared). | # autoclave 50 mL medium with 1.5 % (w/v) agarose (has to be multiplied with the number of chips prepared). | ||
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- | === Chip | + | === Chip Preparation === |
# mix the cooled medium with the cells by inverting gently. | # mix the cooled medium with the cells by inverting gently. | ||
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</center> | </center> | ||
- | == Measurement of | + | == Measurement of Fluorescence == |
Revision as of 14:38, 14 October 2014
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