Team:NCTU Formosa/Notebook/test5

From 2014.igem.org

(Difference between revisions)
Line 74: Line 74:
<div  class="single note">
<div  class="single note">
<p>
<p>
-
8/11</p>
+
<font color="#FF0000"><span style="font-size:1cm;">8/11</span></font><br></p>
<p>
<p>
Transformation of NCTU1 (G1)</p>
Transformation of NCTU1 (G1)</p>
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Transformation of PBAN (G2)</p>
Transformation of PBAN (G2)</p>
<p>
<p>
-
8/12</p>
+
<font color="#FF0000"><span style="font-size:1cm;">8/12</span></font><br></p>
<p>
<p>
Cultivation of [PBAN-4]Kr,[PBAN-5]Krand [PBAN-6]Kr E.coli in LB broth (G1)</p>
Cultivation of [PBAN-4]Kr,[PBAN-5]Krand [PBAN-6]Kr E.coli in LB broth (G1)</p>
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Digestion of [Pcons]Ar (ES) (G2)</p>
Digestion of [Pcons]Ar (ES) (G2)</p>
<p>
<p>
-
8/13</p>
+
<font color="#FF0000"><span style="font-size:1cm;">8/13</span></font><br></p>
<p>
<p>
-
Mini-prep of [PBAN-4] Kr,[PBAN-5] Krand [PBAN-6] Kr (G1)</p>
+
Mini-prep of [PBAN-4] Kr,[PBAN-5] Krand [PBAN-6] Kr (G1)</p>
<p>
<p>
Digestion of [PBAN-4]Kr(EP),[PBAN-5]Kr(EP)and [PBAN-6]Kr(EP) (G1)</p>
Digestion of [PBAN-4]Kr(EP),[PBAN-5]Kr(EP)and [PBAN-6]Kr(EP) (G1)</p>
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Electrophoresis of [Pcons](mini)&(dig ES),digestion of [Pcons](XP),Ligation:[Pcons]+[RBS+BFP+J61048] (G2)</p>
Electrophoresis of [Pcons](mini)&(dig ES),digestion of [Pcons](XP),Ligation:[Pcons]+[RBS+BFP+J61048] (G2)</p>
<p>  
<p>  
-
8/14</p>
+
<font color="#FF0000"><span style="font-size:1cm;">8/14</span></font><br></p>
<p>
<p>
Electrophoresis of plasmid and digestion products of  [PBAN-4] Kr(EP),[PBAN-5] Kr(EP)and [PBAN-6] Kr(EP) (G1)</p>
Electrophoresis of plasmid and digestion products of  [PBAN-4] Kr(EP),[PBAN-5] Kr(EP)and [PBAN-6] Kr(EP) (G1)</p>
<p>
<p>
-
Purification from electrophoresis:[PBAN]No.4&5&6(G1)</p>
+
Purification from electrophoresis of[PBAN]No.4&5&6(G1)</p>
<p>
<p>
Transformation of the ligation product made yesterday</p>
Transformation of the ligation product made yesterday</p>
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Electrophoresis of plasmid and digestion products of [PBAN]No.4&5&6,[Pcons],[Pcons+RBS+BFP+J61048] (G2)</p>
Electrophoresis of plasmid and digestion products of [PBAN]No.4&5&6,[Pcons],[Pcons+RBS+BFP+J61048] (G2)</p>
<p>
<p>
-
8/15</p>
+
<font color="#FF0000"><span style="font-size:1cm;">8/15</span></font><br></p>
<p>
<p>
Digestion of purification products yesterday:[PBAN]No.4&5&6 (EP) (G1)</p>
Digestion of purification products yesterday:[PBAN]No.4&5&6 (EP) (G1)</p>
<p>
<p>
-
Purification from electrophoresis:[PBAN]No.4&5&6</p>
+
Purification from electrophoresis of [PBAN]No.4&5&6</p>
<p>
<p>
Transformation of [B0034]Ar (G2)</p>
Transformation of [B0034]Ar (G2)</p>
<p>  
<p>  
-
8/16</p>
+
<font color="#FF0000"><span style="font-size:1cm;">8/16</span></font><br></p>
<p>
<p>
-
Electrophoresis of the purification products from electrophoresis and digestion products:[PBAN]No.4&5&6 (EP) (G1)</p>
+
Electrophoresis of the purification products from electrophoresis and digestion products of[PBAN]No.4&5&6 (EP) (G1)</p>
<p>
<p>
-
PCR:[B0034]</p>
+
PCR of [B0034]</p>
<p>
<p>
-
Electrophoresis:[PSB1K3](mini)&(dig E),[Pcons](dig SP),PCR products of B0034</p>
+
Electrophoresis of[PSB1K3](mini)&(dig E),[Pcons](dig SP),PCR products of B0034</p>
<p>
<p>
Mini-prep of [B0034]Ar E.coli</p>
Mini-prep of [B0034]Ar E.coli</p>
<p>
<p>
-
Ligation:[Pcons]+[B0034] +PSB1K3</p>
+
Ligation of [Pcons]+[B0034] +PSB1K3</p>
<p>
<p>
Digestion of [BFP](ES)</p>
Digestion of [BFP](ES)</p>
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<div  class="single note">
<div  class="single note">
<p>
<p>
-
8/17</p>
+
<font color="#FF0000"><span style="font-size:1cm;">8/17</span></font><br></p>
<p>
<p>
-
Electrophoresis of the digestion products:[PBAN]No.4&5&6 (EP) (G1)</p>
+
Electrophoresis of the digestion products of[PBAN]No.4&5&6 (EP) (G1)</p>
<br></div>
<br></div>
<div  class="single note">
<div  class="single note">
-
4
+
<font color="#FF0000"><span style="font-size:1cm;">None</span></font><br>
<br></div>
<br></div>
<div  class="single note">
<div  class="single note">
-
5
+
<font color="#FF0000"><span style="font-size:1cm;">None</span></font><br>
<br></div>
<br></div>
</div>
</div>

Revision as of 12:00, 14 October 2014

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8/11

Transformation of NCTU1 (G1)

Transformation of PBAN (G2)

8/12

Cultivation of [PBAN-4]Kr,[PBAN-5]Krand [PBAN-6]Kr E.coli in LB broth (G1)

Cultivation of [RBS+BFP+J61048]Cr ,[T7+RBS+BFP+J61048]Ar Kr

Mini-prep of [Pcons]Ar

Digestion of [Pcons]Ar (ES) (G2)

8/13

Mini-prep of [PBAN-4] Kr,[PBAN-5] Krand [PBAN-6] Kr (G1)

Digestion of [PBAN-4]Kr(EP),[PBAN-5]Kr(EP)and [PBAN-6]Kr(EP) (G1)

Electrophoresis of [Pcons](mini)&(dig ES),digestion of [Pcons](XP),Ligation:[Pcons]+[RBS+BFP+J61048] (G2)

8/14

Electrophoresis of plasmid and digestion products of [PBAN-4] Kr(EP),[PBAN-5] Kr(EP)and [PBAN-6] Kr(EP) (G1)

Purification from electrophoresis of[PBAN]No.4&5&6(G1)

Transformation of the ligation product made yesterday

Electrophoresis of plasmid and digestion products of [PBAN]No.4&5&6,[Pcons],[Pcons+RBS+BFP+J61048] (G2)

8/15

Digestion of purification products yesterday:[PBAN]No.4&5&6 (EP) (G1)

Purification from electrophoresis of [PBAN]No.4&5&6

Transformation of [B0034]Ar (G2)

8/16

Electrophoresis of the purification products from electrophoresis and digestion products of[PBAN]No.4&5&6 (EP) (G1)

PCR of [B0034]

Electrophoresis of[PSB1K3](mini)&(dig E),[Pcons](dig SP),PCR products of B0034

Mini-prep of [B0034]Ar E.coli

Ligation of [Pcons]+[B0034] +PSB1K3

Digestion of [BFP](ES)

Electrophoresis of plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[Pcons])


8/17

Electrophoresis of the digestion products of[PBAN]No.4&5&6 (EP) (G1)


None

None

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