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Team:HokkaidoU Japan/Projects/Length/Method
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<h1> How to synthesize anti-sense constructs</h1> | <h1> How to synthesize anti-sense constructs</h1> | ||
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| + | Anti-sense fragments were synthesized based on BioBrick by PCR. Forward primers are common. Each reverse primers are different. Because of these, we got various length anti-senses. The sides of antisense fragment have restriction enzymes XhoI, NcoI sites. We finished synthesizing anti-sense RNA, we ligated each anti-senses with H-stem construction by restriction enzyme XhoI, NcoI.</p> | ||
<p> | <p> | ||
Revision as of 11:20, 14 October 2014
How to synthesize anti-sense constructs
Anti-sense fragments were synthesized based on BioBrick by PCR. Forward primers are common. Each reverse primers are different. Because of these, we got various length anti-senses. The sides of antisense fragment have restriction enzymes XhoI, NcoI sites. We finished synthesizing anti-sense RNA, we ligated each anti-senses with H-stem construction by restriction enzyme XhoI, NcoI.
Therefore, we got as90, as120. As the same way, we made as30, as60 in H-Stem System and Anti-sense B0034 examination. We performed repression examination by using their 4 anti-sense constructs.
