Team:Saarland/Safety

From 2014.igem.org

(Difference between revisions)
(Prototype team page)
 
(16 intermediate revisions not shown)
Line 1: Line 1:
-
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
+
{{Team:Saarland/14_style}}
 +
{{Team:Saarland/Header}}
 +
{{Team:Saarland/Menu}}
-
 
+
<div id="wikicontent-container">
-
{{CSS/Main}}
+
<div id="wikicontent">
-
 
+
<br>
-
 
+
<h1>Biosafety</h1>
-
<html>
+
-
 
+
-
<!--main content -->
+
-
<table width="70%" align="center">
+
-
 
+
-
 
+
-
<!--welcome box -->
+
-
<tr>
+
-
<td style="border:1px solid black;" colspan="3" align="center" height="150px" bgColor=#FF404B>
+
-
<h1 >WELCOME TO iGEM 2014! </h1>
+
-
<p>Your team has been approved and you are ready to start the iGEM season!
+
-
<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
+
<br>
<br>
-
<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Saarland/Safety&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
+
The term biosafety refers to the minimisation of risks concerning the people working in the laboratory, the people outside the lab and the environment. <i>Bacillus megaterium</i> does not pose any risk by itself, be it to the researchers or to the general public. In the lab, we cultivate <i>B. megaterium</i> in culture medium under controlled conditions.
-
</td>
+
Our main concerns regarding biosafety are:
-
</tr>
+
*toxicity of recombinant proteins
 +
*toxicity of degradation products
 +
*gene transfer (vertical and horizontal)
-
<tr> <td colspan="3"  height="5px"> </td></tr>
+
<h3>Toxicity of recombinant proteins</h3>
-
<!-- end welcome box -->
+
-
<tr>  
+
-
<!--navigation menu -->
+
Recombinant proteins are proteins created by biotechnological means. The gene sequence coding the protein is inserted into a plasmid, which is transformed into the host organism. This recombinant DNA is then integrated into the organism of interest, which should now be able to express the recombinant protein.<br>
-
<td align="center" colspan="3">
+
One of our main concerns is that the recombinant proteins we generate could be toxic, even when our model organisms are not. We have used the synthesised hyaluronan synthase gene (<i>has</i>2) of the naked mole rat, which is a Biosafety level 1 (BSL-1) organism, and <i>B. megaterium</i> (BSL-1 organism).
-
<table  width="100%">
+
<h3>Toxicity of degradation products</h3>
-
<tr heigth="15px"></tr>
+
-
<tr heigth="75px">
+
-
 
+
-
 
+
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
+
-
<a href="https://2014.igem.org/Team:Saarland"style="color:#000000">Home </a> </td>
+
-
 
+
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
-
<a href="https://2014.igem.org/Team:Saarland/Team"style="color:#000000"> Team </a> </td>
+
-
 
+
-
<td style="border:1px solid black;" align="center"  height ="45px"  onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
-
<a href="https://igem.org/Team.cgi?year=2014&team_name=Saarland"style="color:#000000"> Official Team Profile </a></td>
+
-
 
+
-
<td style="border:1px solid black" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
+
-
<a href="https://2014.igem.org/Team:Saarland/Project"style="color:#000000"> Project</a></td>
+
-
 
+
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
-
<a href="https://2014.igem.org/Team:Saarland/Parts"style="color:#000000"> Parts</a></td>
+
-
 
+
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
-
<a href="https://2014.igem.org/Team:Saarland/Modeling"style="color:#000000"> Modeling</a></td>
+
-
 
+
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
+
-
<a href="https://2014.igem.org/Team:Saarland/Notebook"style="color:#000000"> Notebook</a></td>
+
-
 
+
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
-
<a href="https://2014.igem.org/Team:Saarland/Safety"style=" color:#000000"> Safety </a></td>
+
-
 
+
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
-
<a href="https://2014.igem.org/Team:Saarland/Attributions"style="color:#000000"> Attributions </a></td>
+
-
 
+
-
 
+
-
<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
+
-
</tr>
+
-
</table>
+
-
<!--end navigation menu -->
+
-
</tr>
+
-
</tr>
+
-
</td>
+
-
 
+
-
<tr> <td colspan="3"  height="15px"> </td></tr>
+
-
<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
+
-
<tr> <td colspan="3"  height="5px"> </td></tr>
+
-
 
+
-
 
+
-
<!--safety content-->
+
-
<tr><td > <h3> Welcome! </h3></td>
+
-
<td ></td >
+
-
<td > <h3> Timeline</h3></td>
+
-
</tr>
+
-
 
+
-
<tr>
+
-
<td width="45%"  valign="top">
+
-
<p> Visit the <a href="https://2014.igem.org/Safety" >Safety Hub</a> to see this year's safety requirements. The Safety Hub is the central page for everything related to safety in iGEM. You can also go there to learn about general biosafety topics, and how to think about the future implications of your project.</p>
+
 +
Our bacterium does not produce any toxic degradation products. Furthermore the cell culture is autoclaved before disposal, as is all other lab waste.
 +
<h3>Gene transfer</h3>
 +
There are two ways of gene transfer: when the transgene spreads from the transgenic population to a non-transgenic population of the same species, the transfer is called “vertical”; when the transgene spreads to a different species, one speaks of “horizontal” gene transfer.
 +
<br>
 +
<h1>Labsafety</h1>
 +
<br>
 +
We work in a BSL-1 lab, according to the EU directive 2000/54/EG and to the German law for the regulation of genetic engineering “Gesetz zur Regelung der Gentechnik [http://www.gesetze-im-internet.de/gentg/  (GenTG)] ”. This means that no pathogens are used and that by taking general precautionary measures no harm should come to the experimenter.
 +
We have received a safety briefing by our supervisor Prof. Dr. Müller and could ask all potentially occurring questions to Dipl.-Ing. M. Glander, who is responsible for safety in all the labs of the building.<br>
 +
General precautions include, but are not restricted to:
 +
*using labcoats and latex/nitril gloves
 +
*using safety goggles when necessary
 +
*never pipetting with the mouth
 +
*using a fume hood when working with volatile/flammable substances
 +
*storing and disposing of acids and bases separately
<br>
<br>
-
<h3> Edit this page!</h3>
 
-
<p>
 
-
Please use this page to write about anything related to safety in your project. <!--Be sure to talk about both
 
-
<ul>
 
-
<li> <a href=" ">Learn about lab Safety for Today</a></li>
 
-
<li> <a href="">Learn about Safety for the future of your project.</a></li>
 
-
</ul>
 
-
-->
 
-
</p>
 
-
 
-
<h3> Your Lab </h3>
 
-
 
-
 
-
<p> Use this section to tell us about your laboratory. Where is it located? What sort of equipment do you use every day? Have you decorated it for the summer? How do you look wearing a lab coat? Take pictures! Show off your space! </p>
 
-
<!--
 
-
<gallery>
 
-
Image:Example2_Lab_1.png|The building our lab is in!
 
-
Image:Example2_Lab_2.png|The inside of our lab!
 
-
Image:Example2_Lab_3.png|Team Member 3 doing an experiment
 
-
Image:Example2_Lab_4.png|Working in biosafety cabinets
 
-
Image:Example2_Lab_5.png|Team all gloved up and ready for work!
 
-
Image:Example2_Lab_6.png|Equipment that we use to do SCIENCE!
 
-
Image:Example2_Lab_7.png|We decorated this part of our lab
 
-
Image:Example2_Lab_8.png|Whatever else you want
 
-
</gallery>-->
 
-
 
-
</td>
 
-
<td></td>
 
-
 
-
 
-
<td width="45%"  valign="top">
 
-
 
-
 
-
<ul>
 
-
<li> <b>Now :</b>  Read the <a href="https://2014.igem.org/Safety">Safety Hub </a> and learn about safety in iGEM. Ask questions by emailing safety at <i> igem DOT org </i>. </li>
 
-
<li><b>Now - Jamboree:</b> Complete <b>Check-Ins</b> and receive approval before acquiring and using certain materials in your lab</li>
 
-
<li><b>Now - Wiki Freeze:</b> Edit this Safety page to tell us about what you're doing</li>
 
-
<li><b>June 9: </b>Submit the About Our Lab form.</li>
 
-
<li><b>Let us know by June 25 </b>if you will need an extension on the Preliminary Version, or your Preliminary Version will be significantly incomplete.</li>
 
-
<li><b>June 30: </b>Submit the Preliminary Version of the <b>Safety Form</b>.</li>
 
-
<li>Participate in Virtual Open Office Hours to ask questions and discuss safety topics (exact date to be determined).</li>
 
-
<li><b>September 1:</b> Submit the Final Version of the Safety Form.</li>
 
-
<li><b>October: </b> Wiki freeze (exact date to be determined)</li>
 
-
<li><b>October 30 - November 3: </b>GIANT JAMBOREE!</li>
 
-
 
-
</ul>
 
-
</td>
 
-
 
-
</tr>
 
-
 
-
 
-
 
-
 
-
<tr>
 
-
 
-
 
-
 
 +
<h1> Safety Forms </h1>
 +
*[[media:About our lab.pdf|About our lab]]
 +
*[[media:Safety form.pdf|Safety Form ]]
-
</table>
+
<div class="visualClear"> </div>
-
</html>
+
</div>
 +
</div>
 +
{{Team:Saarland/footer}}

Latest revision as of 22:56, 13 October 2014


Biosafety


The term biosafety refers to the minimisation of risks concerning the people working in the laboratory, the people outside the lab and the environment. Bacillus megaterium does not pose any risk by itself, be it to the researchers or to the general public. In the lab, we cultivate B. megaterium in culture medium under controlled conditions. Our main concerns regarding biosafety are:

  • toxicity of recombinant proteins
  • toxicity of degradation products
  • gene transfer (vertical and horizontal)

Toxicity of recombinant proteins

Recombinant proteins are proteins created by biotechnological means. The gene sequence coding the protein is inserted into a plasmid, which is transformed into the host organism. This recombinant DNA is then integrated into the organism of interest, which should now be able to express the recombinant protein.
One of our main concerns is that the recombinant proteins we generate could be toxic, even when our model organisms are not. We have used the synthesised hyaluronan synthase gene (has2) of the naked mole rat, which is a Biosafety level 1 (BSL-1) organism, and B. megaterium (BSL-1 organism).

Toxicity of degradation products

Our bacterium does not produce any toxic degradation products. Furthermore the cell culture is autoclaved before disposal, as is all other lab waste.

Gene transfer

There are two ways of gene transfer: when the transgene spreads from the transgenic population to a non-transgenic population of the same species, the transfer is called “vertical”; when the transgene spreads to a different species, one speaks of “horizontal” gene transfer.

Labsafety


We work in a BSL-1 lab, according to the EU directive 2000/54/EG and to the German law for the regulation of genetic engineering “Gesetz zur Regelung der Gentechnik [http://www.gesetze-im-internet.de/gentg/ (GenTG)] ”. This means that no pathogens are used and that by taking general precautionary measures no harm should come to the experimenter. We have received a safety briefing by our supervisor Prof. Dr. Müller and could ask all potentially occurring questions to Dipl.-Ing. M. Glander, who is responsible for safety in all the labs of the building.
General precautions include, but are not restricted to:

  • using labcoats and latex/nitril gloves
  • using safety goggles when necessary
  • never pipetting with the mouth
  • using a fume hood when working with volatile/flammable substances
  • storing and disposing of acids and bases separately


Safety Forms


Retrieved from "http://2014.igem.org/Team:Saarland/Safety"